6Y25
Streptavidin mutant S112R,K121E with a biotC4-1 cofactor - an artificial iron hydroxylase
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (AAA) | Streptavidin | polymer | 159 | 16639.1 | 1 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | B (AAA) | biotC4-1 cofactor | non-polymer | 667.6 | 1 | Chemie (O6T) | |||
| 3 | C (AAA) | water | water | 18.0 | 66 | Chemie (HOH) |
Sequence modifications
AAA: 15 - 159 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Met 4 | - | expression tag |
| Thr 5 | - | expression tag |
| Gly 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Gln 8 | - | expression tag |
| Gln 9 | - | expression tag |
| Met 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Arg 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Gln 14 | - | expression tag |
| Arg 112 | Ser 136 | engineered mutation |
| Glu 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16639.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 667.6 | |
| All* | Total formula weight | 17306.6 |
