6Y25

Streptavidin mutant S112R,K121E with a biotC4-1 cofactor - an artificial iron hydroxylase

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA	Streptavidin	polymer	159	16639.1	1	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	AAA	biotC4-1 cofactor	non-polymer		667.6	1	Chemie (O6T)
3		water	water		18.0	66	Chemie (HOH)

Sequence modifications

AAA: 15 - 159 (UniProt: P22629)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Gln 14	-	expression tag
Arg 112	Ser 136	engineered mutation
Glu 121	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16639.1
Non-Polymers*	Number of molecules	1
	Total formula weight	667.6
All*	Total formula weight	17306.6

*Water molecules are not included.