6Y1B

X-ray structure of Lactobacillus brevis alcohol dehydrogenase mutant K32A_Q126K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	R-specific alcohol dehydrogenase	polymer	262	27817.3	2	UniProt (Q84EX5) Pfam (PF13561) In PDB	Lactobacillus brevis
2	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5		water	water		18.0	716	Chemie (HOH)

Sequence modifications

A, B: 1 - 251 (UniProt: Q84EX5)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ala 32	Lys 33	engineered mutation
Lys 126	Gln 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55634.6
Non-Polymers*	Number of molecules	4
	Total formula weight	216.8
All*	Total formula weight	55851.4

*Water molecules are not included.