6Y1B
X-ray structure of Lactobacillus brevis alcohol dehydrogenase mutant K32A_Q126K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | R-specific alcohol dehydrogenase | polymer | 262 | 27817.3 | 2 | UniProt (Q84EX5) Pfam (PF13561) | Lactobacillus brevis | |
| 2 | C (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 3 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | F (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | G, H (A, B) | water | water | 18.0 | 716 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 251 (UniProt: Q84EX5)
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| Gly -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 32 | Lys 33 | engineered mutation |
| Lys 126 | Gln 127 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 55634.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 216.8 | |
| All* | Total formula weight | 55851.4 |
