6Y0Z

X-ray structure of Lactobacillus brevis alcohol dehydrogenase mutant Q126K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	R-specific alcohol dehydrogenase	polymer	262	27875.4	1	UniProt (Q84EX5) Pfam (PF13561) In PDB	Lactobacillus brevis
2	A	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
4	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
5	A	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
6		water	water		18.0	286	Chemie (HOH)

Sequence modifications

A: 1 - 251 (UniProt: Q84EX5)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Lys 126	Gln 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27875.4
Non-Polymers*	Number of molecules	13
	Total formula weight	838.3
All*	Total formula weight	28713.8

*Water molecules are not included.