6XWY
Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Red fluorescent protein eqFP611 | polymer | 221 | 24817.1 | 4 | UniProt (Q8ISF8) Pfam (PF01353) | Entacmaea quadricolor (Bubble-tip anemone) | GFP-like chromoprotein |
| 2 | E, H, J (A, B, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 3 | F, G, I (A, B) | MALONIC ACID | non-polymer | 104.1 | 3 | Chemie (MLA) | |||
| 4 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 606 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 3 - 221 (UniProt: Q8ISF8)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Lys 11 | Met 12 | conflict |
| His 21 | Tyr 22 | conflict |
| Glu 30 | Asp 31 | conflict |
| Arg 32 | Asn 33 | conflict |
| Glu 35 | Met 36 | conflict |
| Val 37 | Thr 38 | conflict |
| Ile 45 | Val 46 | conflict |
| Nrq 63 | Met 63 | chromophore |
| Nrq 63 | Tyr 64 | chromophore |
| Nrq 63 | Gly 65 | chromophore |
| Arg 66 | Lys 67 | conflict |
| Tyr 71 | His 72 | conflict |
| Pro 72 | Thr 73 | conflict |
| Ala 73 | Lys 74 | conflict |
| Asp 74 | Gly 75 | conflict |
| Val 101 | Phe 102 | conflict |
| Thr 104 | Met 105 | conflict |
| Glu 113 | Cys 114 | conflict |
| Asn 117 | His 118 | conflict |
| Val 118 | Ala 119 | conflict |
| Arg 121 | Thr 122 | conflict |
| Pro 130 | Ala 131 | conflict |
| Ala 141 | Pro 142 | conflict |
| Asp 142 | Asn 143 | conflict |
| Met 146 | Leu 147 | conflict |
| Leu 157 | Ser 158 | conflict |
| Asp 158 | Gln 159 | conflict |
| Arg 159 | Met 160 | conflict |
| Lys 162 | Asn 163 | conflict |
| His 168 | Tyr 169 | conflict |
| His 170 | Ser 171 | conflict |
| Asn 172 | Ser 173 | conflict |
| Val 174 | Glu 175 | conflict |
| Gly 184 | Glu 185 | conflict |
| Asp 185 | Asn 186 | conflict |
| Ile 186 | Phe 187 | conflict |
| Val 191 | Phe 192 | conflict |
| Ala 193 | Phe 194 | conflict |
| Ile 201 | Leu 202 | conflict |
| Asn 206 | Lys 207 | conflict |
| Thr 208 | Met 209 | conflict |
| Tyr 209 | Phe 210 | conflict |
| Arg 213 | His 214 | conflict |
| Val 215 | His 216 | conflict |
| Tyr 220 | Phe 221 | conflict |
| Ser 221 | Cys 222 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 99268.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 498.4 | |
| All* | Total formula weight | 99766.8 |
