6XQM
Crystal structure of SCLam E144S mutant, a non-specific endo-beta-1,3(4)-glucanase from family GH16, co-crystallized with laminarihexaose, presenting a laminaribiose and a glucose at active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | GH16 family protein | polymer | 269 | 30288.7 | 1 | UniProt (A0A0B5H9B3) UniProt (by SIFTS) (A0ACD6B8S8) | uncultured bacterium | |
| 2 | B (B) | beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G89266VM) | |||
| 3 | C (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 4 | D (A) | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 5 | E (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 6 | F (A) | water | water | 18.0 | 289 | Chemie (HOH) |
Sequence modifications
A: 2 - 266 (UniProt: A0A0B5H9B3)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Met 1 | - | expression tag |
| Ser 144 | Glu 143 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 30288.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 315.2 | |
| All* | Total formula weight | 30946.2 |
