6XLN

Cryo-EM structure of E. coli RNAP-DNA elongation complex 2 (RDe2) in EcmrR-dependent transcription

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli O157:H7	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli O157:H7	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli O157:H7	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli O157:H7	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	N	synthetic non-template strand DNA (54-MER)	polymer	54	16718.7	1		Escherichia coli O157:H7
6	T	synthetic template strand DNA (54-MER)	polymer	54	16563.6	1		Escherichia coli O157:H7
7	R	9-nt RNA transcript	polymer	9	2871.8	1		Escherichia coli O157:H7
8	A, C	CHAPSO	non-polymer		631.9	2	Chemie (1N7)
9	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	425708.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1418.9
All*	Total formula weight	427127.5

*Water molecules are not included.