6XLL
Cryo-EM structure of E. coli RNAP-promoter initial transcribing complex with 5-nt RNA transcript (RPitc-5nt)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli O157:H7 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli O157:H7 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli O157:H7 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli O157:H7 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli O157:H7 | Sigma-70 |
| 6 | G (N) | synthetic non-template strand DNA (54-MER) | polymer | 54 | 16718.7 | 1 | Escherichia coli O157:H7 | ||
| 7 | H (T) | synthetic template strand DNA (54-MER) | polymer | 54 | 16563.6 | 1 | Escherichia coli O157:H7 | ||
| 8 | I (R) | RNA transcript (5-MER) | polymer | 5 | 1746.0 | 1 | Escherichia coli O157:H7 | ||
| 9 | J, K (C) | CHAPSO | non-polymer | 631.9 | 2 | Chemie (1N7) | |||
| 10 | L (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | M, N (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 494935.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1418.9 | |
| All* | Total formula weight | 496353.9 |
