6XL9
Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA transcript (EcmrR-RPitc-3nt)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli O157:H7 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli O157:H7 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli O157:H7 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli O157:H7 | Sigma-70 |
| 5 | F (N) | synthetic non-template strand DNA (54-MER) | polymer | 54 | 16718.7 | 1 | Escherichia coli O157:H7 | ||
| 6 | G (R) | RNA (5'-D(*(GTP))-R(P*AP*G)-3') | polymer | 3 | 1134.6 | 1 | Escherichia coli O157:H7 | ||
| 7 | H (T) | synthetic template strand DNA (54-MER) | polymer | 54 | 16634.6 | 1 | Escherichia coli O157:H7 | ||
| 8 | I, J (G, H) | MerR family transcriptional regulator EcmrR | polymer | 268 | 31394.2 | 2 | Escherichia coli O157:H7 | ||
| 9 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | M, O (D, R) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 11 | N, P, Q (F, G, H) | TETRAPHENYLANTIMONIUM ION | non-polymer | 430.2 | 3 | Chemie (118) | |||
| 12 | R, S, T (C, D, T) | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 546933.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1470.0 | |
| All* | Total formula weight | 548403.5 |
