6XJ5

Carboxypeptidase G2 modified with a versatile bioconjugate for metalloprotein design

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	Carboxypeptidase G2	polymer	411	44006.9	4	UniProt (P06621) Pfam (PF01546) Pfam (PF07687) In PDB	Pseudomonas sp. (strain RS-16)	CPDG2,Folate hydrolase G2,Glutamate carboxypeptidase,Pteroylmonoglutamic acid hydrolase G2
2	B, D, F, H	Carboxypeptidase G2	polymer	411	44195.1	4	UniProt (P06621) Pfam (PF01546) Pfam (PF07687) In PDB	Pseudomonas sp. (strain RS-16)	CPDG2,Folate hydrolase G2,Glutamate carboxypeptidase,Pteroylmonoglutamic acid hydrolase G2
3	F, D, G, H, A...	ZINC ION	non-polymer		65.4	56	Chemie (ZN)
4	C	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5		water	water		18.0	120	Chemie (HOH)

Sequence modifications

A, C, E, G: 24 - 415 (UniProt: P06621)

PDB	External Database	Details
Met 5	-	initiating methionine
Gly 6	-	expression tag
Ser 7	-	expression tag
Asp 8	-	expression tag
Lys 9	-	expression tag
Ile 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Glu 17	-	expression tag
Asn 18	-	expression tag
Leu 19	-	expression tag
Tyr 20	-	expression tag
Phe 21	-	expression tag
Gln 22	-	expression tag
Gly 23	-	expression tag
Cys 203	Ser 203	engineered mutation

B, D, F, H: 24 - 415 (UniProt: P06621)

PDB	External Database	Details
Met 5	-	initiating methionine
Gly 6	-	expression tag
Ser 7	-	expression tag
Asp 8	-	expression tag
Lys 9	-	expression tag
Ile 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Glu 17	-	expression tag
Asn 18	-	expression tag
Leu 19	-	expression tag
Tyr 20	-	expression tag
Phe 21	-	expression tag
Gln 22	-	expression tag
Gly 23	-	expression tag
V44 203	Ser 203	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	352807.9
Non-Polymers*	Number of molecules	58
	Total formula weight	3855.0
All*	Total formula weight	356662.9

*Water molecules are not included.