6XIW

Cryo-EM structure of the sodium leak channel NALCN-FAM155A complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sodium leak channel non-selective protein	polymer	1794	206341.6	1	UniProt (Q8IZF0) Pfam (PF00520)	Homo sapiens (Human)	CanIon,Voltage gated channel-like protein 1
2	B (B)	Transmembrane protein FAM155A	polymer	483	54205.0	1	UniProt (B1AL88)	Homo sapiens (Human)
3	C, D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4	E, F, G, H, I (A)	(1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		720.0	5	Chemie (PEV)
5	J, K (A)	(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE	non-polymer		749.0	2	Chemie (PGV)
6	L, M, N (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	3	Chemie (Y01)
7	O, P (A, B)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 1738 (UniProt: Q8IZF0)

PDB	External Database	Details
Gly 1739	-	expression tag
Gly 1740	-	expression tag
Gly 1741	-	expression tag
Ser 1742	-	expression tag
Gly 1743	-	expression tag
Gly 1744	-	expression tag
Trp 1745	-	expression tag
Ser 1746	-	expression tag
His 1747	-	expression tag
Pro 1748	-	expression tag
Gln 1749	-	expression tag
Phe 1750	-	expression tag
Glu 1751	-	expression tag
Lys 1752	-	expression tag
Gly 1753	-	expression tag
Gly 1754	-	expression tag
Gly 1755	-	expression tag
Ser 1756	-	expression tag
Gly 1757	-	expression tag
Gly 1758	-	expression tag
Gly 1759	-	expression tag
Ser 1760	-	expression tag
Gly 1761	-	expression tag
Gly 1762	-	expression tag
Ser 1763	-	expression tag
Ala 1764	-	expression tag
Trp 1765	-	expression tag
Ser 1766	-	expression tag
His 1767	-	expression tag
Pro 1768	-	expression tag
Gln 1769	-	expression tag
Phe 1770	-	expression tag
Glu 1771	-	expression tag
Lys 1772	-	expression tag
Gly 1773	-	expression tag
Ser 1774	-	expression tag
Gly 1775	-	expression tag
Asp 1776	-	expression tag
Tyr 1777	-	expression tag
Lys 1778	-	expression tag
Asp 1779	-	expression tag
Asp 1780	-	expression tag
Asp 1781	-	expression tag
Asp 1782	-	expression tag
Lys 1783	-	expression tag
Gly 1784	-	expression tag
Asn 1785	-	expression tag
Ser 1786	-	expression tag
Asp 1787	-	expression tag
Tyr 1788	-	expression tag
Lys 1789	-	expression tag
Asp 1790	-	expression tag
Asp 1791	-	expression tag
Asp 1792	-	expression tag
Asp 1793	-	expression tag
Lys 1794	-	expression tag

B: 1 - 458 (UniProt: B1AL88)

PDB	External Database	Details
Gly 459	-	expression tag
Gly 460	-	expression tag
Ser 461	-	expression tag
Gly 462	-	expression tag
Gly 463	-	expression tag
Ser 464	-	expression tag
Asp 465	-	expression tag
Tyr 466	-	expression tag
Lys 467	-	expression tag
Asp 468	-	expression tag
Asp 469	-	expression tag
Asp 470	-	expression tag
Asp 471	-	expression tag
Lys 472	-	expression tag
Gly 473	-	expression tag
Asn 474	-	expression tag
Ser 475	-	expression tag
Asp 476	-	expression tag
Tyr 477	-	expression tag
Lys 478	-	expression tag
Asp 479	-	expression tag
Asp 480	-	expression tag
Asp 481	-	expression tag
Asp 482	-	expression tag
Lys 483	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	260546.6
Non-Polymers*	Number of molecules	12
	Total formula weight	7000.7
All*	Total formula weight	267547.3

*Water molecules are not included.