6XEX

Structure of Serratia marcescens 2,3-butanediol dehydrogenase mutant Q247A/V139Q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	2,3-butanediol dehydrogenase	polymer	264	27911.7	2	UniProt (H9XP47) Pfam (PF13561) In PDB	Serratia marcescens	3-oxoacyl-ACP reductase,SDR family oxidoreductase
2	A	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	1	Chemie (NAD)
3	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	A, B	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5		water	water		18.0	542	Chemie (HOH)

Sequence modifications

A, B: 2 - 251 (UniProt: H9XP47)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Gln 139	Val 139	engineered mutation
Ala 247	Gln 247	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55823.4
Non-Polymers*	Number of molecules	8
	Total formula weight	1019.7
All*	Total formula weight	56843.2

*Water molecules are not included.