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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

6XDQ

Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site

This is a non-PDB format compatible entry.
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1050S ribosomal protein L21polymer10311586.41UniProt (D7ZEN7)
UniProt (by SIFTS) (P0AG48)
Pfam (PF00829)
In PDB		Escherichia coli
2150S ribosomal protein L22polymer11012253.41UniProt (D7Z9G0)
UniProt (by SIFTS) (P61175)
Pfam (PF00237)
In PDB		Escherichia coli
3250S ribosomal protein L23polymer10011222.21UniProt (D7X2Z9)
UniProt (by SIFTS) (P0ADZ0)
Pfam (PF00276)
In PDB		Escherichia coli
4350S ribosomal protein L24polymer10411339.31UniProt (D7Z9G6)
UniProt (by SIFTS) (P60624)
Pfam (PF17136)
Pfam (PF00467)
In PDB		Escherichia coli
5450S ribosomal protein L25polymer9410713.51UniProt (D7XH79)
UniProt (by SIFTS) (P68919)
Pfam (PF01386)
In PDB		Escherichia coli
65NT DNApolymer3611042.11synthetic construct
76T DNApolymer3610956.01synthetic construct
87mRNA with 30 nt long spacerpolymer4614443.11synthetic construct
9950S ribosomal protein L10polymer16517736.61UniProt (D7ZA73)
UniProt (by SIFTS) (P0A7J3)
Pfam (PF00466)
In PDB		Escherichia coli
10A, BE-site and P-site tRNA (fMet)polymer7624496.62GenBank (1384458579)Escherichia coli
11AADNA-directed RNA polymerase subunit betapolymer1342150820.91UniProt (P0A8V4)
UniProt (by SIFTS) (P0A8V2)
Pfam (PF04563)
Pfam (PF04561)
Pfam (PF04565)
Pfam (PF10385)
Pfam (PF00562)
Pfam (PF04560)
In PDB		Escherichia coliRNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
12ABTranscription termination/antitermination protein NusGpolymer18120560.51UniProt (V8K3D7)
UniProt (by SIFTS) (P0AFG0)
Pfam (PF02357)
Pfam (PF00467)
In PDB		Escherichia coli
13AC, ADDNA-directed RNA polymerase subunit alphapolymer32936558.72UniProt (A0A073G207)
UniProt (by SIFTS) (P0A7Z4)
Pfam (PF01193)
Pfam (PF01000)
Pfam (PF03118)
In PDB		Escherichia coliRNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
14AEDNA-directed RNA polymerase subunit beta'polymer1407155366.81UniProt (A0A4S1NBU2)
UniProt (by SIFTS) (P0A8T7)
Pfam (PF05000)
Pfam (PF04998)
Pfam (PF04997)
Pfam (PF00623)
Pfam (PF04983)
In PDB		Escherichia coliRNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
15AFDNA-directed RNA polymerase subunit omegapolymer9110249.51UniProt (P0A802)
UniProt (by SIFTS) (P0A800)
Pfam (PF01192)
In PDB		Escherichia coliRNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
16AGTranscription termination/antitermination protein NusApolymer49554932.71UniProt (U9XV73)
UniProt (by SIFTS) (P0AFF6)
Pfam (PF14520)
Pfam (PF08529)
Pfam (PF00575)
Pfam (PF13184)
In PDB		Escherichia coli
17C30S ribosomal protein S18polymer759005.51UniProt (A0A0E2KXL3)
UniProt (by SIFTS) (P0A7T7)
Pfam (PF01084)
In PDB		Escherichia coli
18D16S rRNApolymer1542499690.01GenBank (1732209707)Escherichia coli
19E30S ribosomal protein S20polymer879708.51UniProt (D7ZAS2)
UniProt (by SIFTS) (P0A7U7)
Pfam (PF01649)
In PDB		Escherichia coli
20F30S ribosomal protein S21polymer718524.01UniProt (A0A0E2L2J1)
UniProt (by SIFTS) (P68679)
Pfam (PF01165)
In PDB		Escherichia coli
21G30S ribosomal protein S2polymer24126781.71UniProt (D7ZK99)
UniProt (by SIFTS) (P0A7V0)
Pfam (PF00318)
In PDB		Escherichia coli
22H30S ribosomal protein S1polymer55761238.91UniProt (D6HUW2)
UniProt (by SIFTS) (P0AG67)
Pfam (PF00575)
In PDB		Escherichia coli
23I30S ribosomal protein S3polymer23326031.31UniProt (B7MCS9)
UniProt (by SIFTS) (P0A7V3)
Pfam (PF07650)
Pfam (PF00189)
In PDB		Escherichia coli
24J30S ribosomal protein S4polymer20623514.21UniProt (D7Z9H7)
UniProt (by SIFTS) (P0A7V8)
Pfam (PF00163)
Pfam (PF01479)
In PDB		Escherichia coli
25K30S ribosomal protein S5polymer16717629.41UniProt (D7Z9H2)
UniProt (by SIFTS) (P0A7W1)
Pfam (PF00333)
Pfam (PF03719)
In PDB		Escherichia coli
26L30S ribosomal protein S6polymer13515727.51UniProt (P02358)
Pfam (PF01250)
In PDB		Escherichia coliSmall ribosomal subunit protein bS6
27M30S ribosomal protein S7polymer17920055.21UniProt (A0A5Q2GFB5)
UniProt (by SIFTS) (P02359)
Pfam (PF00177)
In PDB		Escherichia coli
28N30S ribosomal protein S8polymer13014146.61UniProt (D7Z9G9)
UniProt (by SIFTS) (P0A7W7)
Pfam (PF00410)
In PDB		Escherichia coli
29O30S ribosomal protein S9polymer13014886.31UniProt (D7ZES9)
UniProt (by SIFTS) (P0A7X3)
Pfam (PF00380)
In PDB		Escherichia coli
30P30S ribosomal protein S10polymer10311755.61UniProt (D7Z9F4)
UniProt (by SIFTS) (P0A7R5)
Pfam (PF00338)
In PDB		Escherichia coli
31Q30S ribosomal protein S11polymer12913871.01UniProt (B7MCR3)
UniProt (by SIFTS) (P0A7R9)
Pfam (PF00411)
In PDB		Escherichia coli
32R30S ribosomal protein S12polymer12413768.21UniProt (D7ZAN0)
UniProt (by SIFTS) (P0A7S3)
Pfam (PF00164)
In PDB		Escherichia coli
33S30S ribosomal protein S14polymer10111606.61UniProt (D7Z9G8)
UniProt (by SIFTS) (P0AG59)
Pfam (PF00253)
In PDB		Escherichia coli
34T30S ribosomal protein S15polymer8910290.81UniProt (D7ZEL4)
UniProt (by SIFTS) (P0ADZ4)
Pfam (PF00312)
In PDB		Escherichia coli
35U30S ribosomal protein S16polymer829207.61UniProt (B7MIU7)
UniProt (by SIFTS) (P0A7T3)
Pfam (PF00886)
In PDB		Escherichia coli
36V30S ribosomal protein S17polymer849724.51UniProt (D7Z9G4)
UniProt (by SIFTS) (P0AG63)
Pfam (PF00366)
In PDB		Escherichia coli
37W30S ribosomal protein S19polymer9210455.41UniProt (D7Z9F9)
UniProt (by SIFTS) (P0A7U3)
Pfam (PF00203)
In PDB		Escherichia coli
38X30S ribosomal protein S13polymer11813128.51UniProt (H4UQ02)
UniProt (by SIFTS) (P0A7S9)
Pfam (PF00416)
In PDB		Escherichia coli
39Y50S ribosomal protein L11polymer14214894.41UniProt (D7ZA71)
UniProt (by SIFTS) (P0A7J7)
Pfam (PF03946)
Pfam (PF00298)
In PDB		Escherichia coli
40Z50S ribosomal protein L7/L12polymer12112309.21UniProt (H4KQ39)
UniProt (by SIFTS) (P0A7K2)
Pfam (PF16320)
Pfam (PF00542)
In PDB		Escherichia coli
41a23S rRNApolymer2904941635.41GenBank (937521852)Escherichia coli
42b50S ribosomal protein L27polymer859146.51UniProt (P0A7L8)
Pfam (PF01016)
In PDB		Escherichia coliLarge ribosomal subunit protein bL27
43c50S ribosomal protein L28polymer789027.61UniProt (S0YB49)
UniProt (by SIFTS) (P0A7M2)
Pfam (PF00830)
In PDB		Escherichia coli
44d5S rRNApolymer12038790.11GenBank (1273279017)Escherichia coli
45e50S ribosomal protein L29polymer637286.51UniProt (D7Z9G3)
UniProt (by SIFTS) (P0A7M6)
Pfam (PF00831)
In PDB		Escherichia coli
46f50S ribosomal protein L30polymer596554.81UniProt (D7Z9H3)
UniProt (by SIFTS) (P0AG51)
Pfam (PF00327)
In PDB		Escherichia coli
47g50S ribosomal protein L31polymer707887.11UniProt (D7ZFP6)
UniProt (by SIFTS) (P0A7M9)
Pfam (PF01197)
In PDB		Escherichia coli
48h50S ribosomal protein L2polymer27329923.61UniProt (P60422)
Pfam (PF00181)
Pfam (PF03947)
In PDB		Escherichia coliLarge ribosomal subunit protein uL2
49i50S ribosomal protein L32polymer576463.41UniProt (D7ZGG2)
UniProt (by SIFTS) (P0A7N4)
Pfam (PF01783)
In PDB		Escherichia coli
50j50S ribosomal protein L3polymer20922277.51UniProt (P60438)
Pfam (PF00297)
In PDB		Escherichia coliLarge ribosomal subunit protein uL3
51k50S ribosomal protein L33polymer556388.61UniProt (D7Z9P0)
UniProt (by SIFTS) (P0A7N9)
Pfam (PF00471)
In PDB		Escherichia coli
52l50S ribosomal protein L4polymer20122121.61UniProt (D7Z9F6)
UniProt (by SIFTS) (P60723)
Pfam (PF00573)
In PDB		Escherichia coli
53m50S ribosomal protein L34polymer465397.51UniProt (B7MGC4)
UniProt (by SIFTS) (P0A7P5)
Pfam (PF00468)
In PDB		Escherichia coli
54n50S ribosomal protein L5polymer17920333.61UniProt (D7Z9G7)
UniProt (by SIFTS) (P62399)
Pfam (PF00281)
Pfam (PF00673)
In PDB		Escherichia coli
55o50S ribosomal protein L35polymer657313.01UniProt (D7Z814)
UniProt (by SIFTS) (P0A7Q1)
Pfam (PF01632)
In PDB		Escherichia coli
56p50S ribosomal protein L6polymer17718932.81UniProt (D7Z9H0)
UniProt (by SIFTS) (P0AG55)
Pfam (PF00347)
In PDB		Escherichia coli
57q50S ribosomal protein L36polymer384377.41UniProt (A0A0E2L017)
UniProt (by SIFTS) (P0A7Q6)
Pfam (PF00444)
In PDB		Escherichia coli
58r50S ribosomal protein L9polymer14915789.01UniProt (D7ZI15)
UniProt (by SIFTS) (P0A7R1)
Pfam (PF01281)
Pfam (PF03948)
In PDB		Escherichia coli
59s50S ribosomal protein L13polymer14216050.61UniProt (D7ZET0)
UniProt (by SIFTS) (P0AA10)
Pfam (PF00572)
In PDB		Escherichia coli
60t50S ribosomal protein L14polymer12313565.11UniProt (D7Z9G5)
UniProt (by SIFTS) (P0ADY3)
Pfam (PF00238)
In PDB		Escherichia coli
61u50S ribosomal protein L15polymer14415008.51UniProt (A0A037Y8L6)
UniProt (by SIFTS) (P02413)
Pfam (PF00828)
In PDB		Escherichia coli
62v50S ribosomal protein L16polymer13615312.31UniProt (P0ADY7)
Pfam (PF00252)
In PDB		Escherichia coliLarge ribosomal subunit protein uL16
63w50S ribosomal protein L17polymer12714393.71UniProt (D6I1Y5)
UniProt (by SIFTS) (P0AG44)
Pfam (PF01196)
In PDB		Escherichia coli
64x50S ribosomal protein L18polymer11712794.71UniProt (D7Z9H1)
UniProt (by SIFTS) (P0C018)
Pfam (PF00861)
In PDB		Escherichia coli
65y50S ribosomal protein L19polymer11513159.31UniProt (D7ZKU3)
UniProt (by SIFTS) (P0A7K6)
Pfam (PF01245)
In PDB		Escherichia coli
66z50S ribosomal protein L20polymer11813528.01UniProt (L3CCT0)
UniProt (by SIFTS) (P0A7L3)
Pfam (PF00453)
In PDB		Escherichia coli
67AEMAGNESIUM IONnon-polymer24.31Chemie (MG)
68AEZINC IONnon-polymer65.42Chemie (ZN)
Sequence modifications
AE: 1 - 1407 (UniProt: A0A4S1NBU2)
PDBExternal DatabaseDetails
Val 1384Met 1384variant
a: 1 - 2904 (GenBank: 937521852)
PDBExternal DatabaseDetails
A 887U 472128variant
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains68
Total formula weight2796742.1
Non-Polymers*Number of molecules3
Total formula weight155.1
All*Total formula weight2796897.2
*Water molecules are not included.

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PDB entries from 2024-07-17

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