6XBD

Cryo-EM structure of MlaFEDB in nanodiscs with phospholipid substrates

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Phospholipid ABC transporter-binding protein MlaD	polymer	201	21937.7	6	UniProt (H4UPP8)	Escherichia coli DEC6A
2	G, H (G, H)	Phospholipid ABC transporter permease protein MlaE	polymer	260	27885.2	2	UniProt (H4UPP9)	Escherichia coli DEC6A
3	I, J (I, J)	Phospholipid transport system ATP-binding protein MlaF	polymer	269	29128.8	2	UniProt (H4UPQ0)	Escherichia coli DEC6A
4	K, L (K, L)	Phospholipid ABC transporter-binding protein MlaB	polymer	97	10690.3	2	UniProt (H4UPP6)	Escherichia coli K-12
5	M, N (M, N)	MSP1D1	polymer	164	13975.2	2		Homo sapiens
6	O, P (G)	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	2	Chemie (PEF)

Sequence modifications

A, B, C, D, E, F: 1 - 183 (UniProt: H4UPP8)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Gln -10	-	expression tag
His -9	-	expression tag
Gln -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	294985.0
Non-Polymers*	Number of molecules	2
	Total formula weight	1383.9
All*	Total formula weight	296368.9

*Water molecules are not included.