6X7F
Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (0) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (D7ZEN7) Pfam (PF00829) UniProt (by SIFTS) (P0AG48) | Escherichia coli | |
| 2 | B (1) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (D7Z9G0) Pfam (PF00237) UniProt (by SIFTS) (P61175) | Escherichia coli | |
| 3 | C (2) | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (D7X2Z9) Pfam (PF00276) UniProt (by SIFTS) (P0ADZ0) | Escherichia coli | |
| 4 | D (3) | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (D7Z9G6) Pfam (PF00467) Pfam (PF17136) UniProt (by SIFTS) (P60624) | Escherichia coli | |
| 5 | E (4) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (D7XH79) Pfam (PF01386) UniProt (by SIFTS) (P68919) | Escherichia coli | |
| 6 | F (5) | NT DNA | polymer | 36 | 11042.1 | 1 | synthetic construct | ||
| 7 | G (6) | T DNA | polymer | 36 | 10956.0 | 1 | synthetic construct | ||
| 8 | H (7) | mRNA with 24 nt long spacer | polymer | 41 | 12912.3 | 1 | synthetic construct | ||
| 9 | I (9) | 50S ribosomal protein L10 | polymer | 165 | 17736.6 | 1 | UniProt (D7ZA73) Pfam (PF00466) UniProt (by SIFTS) (P0A7J3) | Escherichia coli | |
| 10 | J, R (A, B) | E-site and P-site tRNA (fMet) | polymer | 76 | 24496.6 | 2 | GenBank (1848954948) | Escherichia coli | |
| 11 | K (AA) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 12 | L (AB) | Transcription termination/antitermination protein NusG | polymer | 181 | 20560.5 | 1 | UniProt (U9XYQ6) Pfam (PF02357) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0) | Escherichia coli | |
| 13 | M, N (AC, AD) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 14 | O (AE) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 15 | P (AF) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 16 | Q (AG) | Transcription termination/antitermination protein NusA | polymer | 495 | 54932.7 | 1 | UniProt (U9XV73) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520) UniProt (by SIFTS) (P0AFF6) | Escherichia coli | |
| 17 | S (C) | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (A0A0E2KXL3) Pfam (PF01084) UniProt (by SIFTS) (P0A7T7) | Escherichia coli | |
| 18 | T (D) | 16S rRNA | polymer | 1542 | 499690.0 | 1 | GenBank (1732209707) | Escherichia coli | |
| 19 | U (E) | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (D7ZAS2) Pfam (PF01649) UniProt (by SIFTS) (P0A7U7) | Escherichia coli | |
| 20 | V (F) | 30S ribosomal protein S21 | polymer | 71 | 8524.0 | 1 | UniProt (A0A0E2L2J1) Pfam (PF01165) UniProt (by SIFTS) (P68679) | Escherichia coli | |
| 21 | W (G) | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (D7ZK99) Pfam (PF00318) UniProt (by SIFTS) (P0A7V0) | Escherichia coli | |
| 22 | X (H) | 30S ribosomal protein S1 | polymer | 557 | 61238.9 | 1 | UniProt (D6HUW2) Pfam (PF00575) UniProt (by SIFTS) (P0AG67) | Escherichia coli | |
| 23 | Y (I) | 30S ribosomal protein S3 | polymer | 233 | 26031.3 | 1 | UniProt (B7MCS9) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P0A7V3) | Escherichia coli | |
| 24 | Z (J) | 30S ribosomal protein S4 | polymer | 206 | 23514.2 | 1 | UniProt (D7Z9H7) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P0A7V8) | Escherichia coli | |
| 25 | AA (K) | 30S ribosomal protein S5 | polymer | 167 | 17629.4 | 1 | UniProt (D7Z9H2) Pfam (PF00333) Pfam (PF03719) UniProt (by SIFTS) (P0A7W1) | Escherichia coli | |
| 26 | BA (L) | 30S ribosomal protein S6 | polymer | 135 | 15727.5 | 1 | UniProt (P02358) Pfam (PF01250) | Escherichia coli | Small ribosomal subunit protein bS6 |
| 27 | CA (M) | 30S ribosomal protein S7 | polymer | 179 | 20055.2 | 1 | UniProt (A0A5Q2GFB5) Pfam (PF00177) UniProt (by SIFTS) (P02359) | Escherichia coli | |
| 28 | DA (N) | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (D7Z9G9) Pfam (PF00410) UniProt (by SIFTS) (P0A7W7) | Escherichia coli | |
| 29 | EA (O) | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (D7ZES9) Pfam (PF00380) UniProt (by SIFTS) (P0A7X3) | Escherichia coli | |
| 30 | FA (P) | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (D7Z9F4) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5) | Escherichia coli | |
| 31 | GA (Q) | 30S ribosomal protein S11 | polymer | 129 | 13871.0 | 1 | UniProt (B7MCR3) Pfam (PF00411) UniProt (by SIFTS) (P0A7R9) | Escherichia coli | |
| 32 | HA (R) | 30S ribosomal protein S12 | polymer | 124 | 13768.2 | 1 | UniProt (D7ZAN0) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3) | Escherichia coli | |
| 33 | IA (S) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (D7Z9G8) Pfam (PF00253) UniProt (by SIFTS) (P0AG59) | Escherichia coli | |
| 34 | JA (T) | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (D7ZEL4) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4) | Escherichia coli | |
| 35 | KA (U) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (B7MIU7) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3) | Escherichia coli | |
| 36 | LA (V) | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (D7Z9G4) Pfam (PF00366) UniProt (by SIFTS) (P0AG63) | Escherichia coli | |
| 37 | MA (W) | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (D7Z9F9) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3) | Escherichia coli | |
| 38 | NA (X) | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (H4UQ02) Pfam (PF00416) UniProt (by SIFTS) (P0A7S9) | Escherichia coli | |
| 39 | OA (Y) | 50S ribosomal protein L11 | polymer | 142 | 14894.4 | 1 | UniProt (D7ZA71) Pfam (PF03946) Pfam (PF00298) UniProt (by SIFTS) (P0A7J7) | Escherichia coli | |
| 40 | PA (Z) | 50S ribosomal protein L7/L12 | polymer | 121 | 12309.2 | 1 | UniProt (V0AEG5) Pfam (PF16320) Pfam (PF00542) UniProt (by SIFTS) (P0A7K2) | Escherichia coli | |
| 41 | QA (a) | 23S rRNA | polymer | 2904 | 941635.4 | 1 | GenBank (937521852) | Escherichia coli | |
| 42 | RA (b) | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli | Large ribosomal subunit protein bL27 |
| 43 | SA (c) | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (S0YB49) Pfam (PF00830) UniProt (by SIFTS) (P0A7M2) | Escherichia coli | |
| 44 | TA (d) | 5S rRNA | polymer | 120 | 38790.1 | 1 | GenBank (1273279017) | Escherichia coli | |
| 45 | UA (e) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (D7Z9G3) Pfam (PF00831) UniProt (by SIFTS) (P0A7M6) | Escherichia coli | |
| 46 | VA (f) | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (D7Z9H3) Pfam (PF00327) UniProt (by SIFTS) (P0AG51) | Escherichia coli | |
| 47 | WA (g) | 50S ribosomal protein L31 | polymer | 70 | 7887.1 | 1 | UniProt (D7ZFP6) Pfam (PF01197) UniProt (by SIFTS) (P0A7M9) | Escherichia coli | |
| 48 | XA (h) | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | Large ribosomal subunit protein uL2 |
| 49 | YA (i) | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (D7ZGG2) Pfam (PF01783) UniProt (by SIFTS) (P0A7N4) | Escherichia coli | |
| 50 | ZA (j) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli | Large ribosomal subunit protein uL3 |
| 51 | AB (k) | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (D7Z9P0) Pfam (PF00471) UniProt (by SIFTS) (P0A7N9) | Escherichia coli | |
| 52 | BB (l) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (D7Z9F6) Pfam (PF00573) UniProt (by SIFTS) (P60723) | Escherichia coli | |
| 53 | CB (m) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (B7MGC4) Pfam (PF00468) UniProt (by SIFTS) (P0A7P5) | Escherichia coli | |
| 54 | DB (n) | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (D7Z9G7) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (P62399) | Escherichia coli | |
| 55 | EB (o) | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (D7Z814) Pfam (PF01632) UniProt (by SIFTS) (P0A7Q1) | Escherichia coli | |
| 56 | FB (p) | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (D7Z9H0) Pfam (PF00347) UniProt (by SIFTS) (P0AG55) | Escherichia coli | |
| 57 | GB (q) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A0E2L017) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6) | Escherichia coli | |
| 58 | HB (r) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (D7ZI15) Pfam (PF01281) Pfam (PF03948) UniProt (by SIFTS) (P0A7R1) | Escherichia coli | |
| 59 | IB (s) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (D7ZET0) Pfam (PF00572) UniProt (by SIFTS) (P0AA10) | Escherichia coli | |
| 60 | JB (t) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (D7Z9G5) Pfam (PF00238) UniProt (by SIFTS) (P0ADY3) | Escherichia coli | |
| 61 | KB (u) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A037Y8L6) Pfam (PF00828) UniProt (by SIFTS) (P02413) | Escherichia coli | |
| 62 | LB (v) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 63 | MB (w) | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (D6I1Y5) Pfam (PF01196) UniProt (by SIFTS) (P0AG44) | Escherichia coli | |
| 64 | NB (x) | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (D7Z9H1) Pfam (PF00861) UniProt (by SIFTS) (P0C018) | Escherichia coli | |
| 65 | OB (y) | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (D7ZKU3) Pfam (PF01245) UniProt (by SIFTS) (P0A7K6) | Escherichia coli | |
| 66 | PB (z) | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (B7MAS6) Pfam (PF00453) UniProt (by SIFTS) (P0A7L3) | Escherichia coli | |
| 67 | QB (AE) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 68 | RB, SB (AE) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 76 (GenBank: 1848954948)
AE: 1 - 1407 (UniProt: A0A4S1NBU2)
a: 1 - 2904 (GenBank: 937521852)
| PDB | External Database | Details |
|---|---|---|
| - | U 4104771 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Val 1384 | Met 1384 | variant |
| PDB | External Database | Details |
|---|---|---|
| A 887 | U 472128 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 68 |
| Total formula weight | 2795211.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 2795366.4 |
