6X59

The mouse cGAS catalytic domain binding to human assembled nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15257.8	2	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (Human)	H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13990.3	2	UniProt (P0C0S8) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	H2A.1,Histone H2A/ptl
4	D, H (D, H)	Histone H2B type 1-C/E/F/G/I	polymer	125	13796.0	2	UniProt (P62807) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l
5	I (I)	DNA	polymer	147	45145.8	1		Homo sapiens
6	J (J)	DNA	polymer	147	45604.0	1		Homo sapiens
7	K (K)	Cyclic GMP-AMP synthase	polymer	372	43647.4	1	UniProt (Q8C6L5) Pfam (PF03281) Pfam (PF20266)	Mus musculus (Mouse)	m-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1
8	L (K)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, E: 1 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 110	Cys 111	conflict

D, H: 2 - 125 (UniProt: P62807)

PDB	External Database	Details
Ser 1	-	expression tag

K: 142 - 507 (UniProt: Q8C6L5)

PDB	External Database	Details
Gly 136	-	expression tag
Ser 137	-	expression tag
Glu 138	-	expression tag
Phe 139	-	expression tag
Glu 140	-	expression tag
Leu 141	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	243011.9
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	243077.3

*Water molecules are not included.