6X0M

Bridging of double-strand DNA break activates PARP2/HPF1 to modify chromatin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (O, o)	Histone PARylation factor 1	polymer	356	40598.3	2	UniProt (Q9NWY4) Pfam (PF10228)	Homo sapiens (Human)
2	B, D (P, p)	Poly [ADP-ribose] polymerase 2	polymer	590	67072.5	2	UniProt (Q9UGN5) Pfam (PF05406) Pfam (PF02877) Pfam (PF00644)	Homo sapiens (Human)	hPARP-2,ADP-ribosyltransferase diphtheria toxin-like 2,ARTD2,DNA ADP-ribosyltransferase PARP2,NAD(+) ADP-ribosyltransferase 2,ADPRT-2,Poly[ADP-ribose] synthase 2,pADPRT-2,Protein poly-ADP-ribosyltransferase PARP2
3	E, G (I, i)	DNA (167-MER)	polymer	167	51776.0	2		synthetic construct
4	F, H (J, j)	DNA (167-MER)	polymer	167	51330.7	2		synthetic construct

Sequence modifications

O, o: 1 - 346 (UniProt: Q9NWY4)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Lys 174	Arg 174	variant

P, p: 1 - 570 (UniProt: Q9UGN5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	421555.0
All*	Total formula weight	421555.0

*Water molecules are not included.