6WZ6
Complex of mutant (K173M) of Pseudomonas 7A Glutaminase-Asparaginase with L-Glu at pH 5. Covalent acyl-enzyme intermediate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glutaminase-asparaginase | polymer | 337 | 36198.0 | 4 | UniProt (Q88K39) Pfam (PF00710) Pfam (PF17763) | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) | L-ASNase/L-GLNase,L-asparagine/L-glutamine amidohydrolase |
| 2 | E, G, H, J (A, B, C, D) | GLUTAMIC ACID | non-polymer | 147.1 | 4 | Chemie (GLU) | |||
| 3 | F, I, K (A, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 4 | L, M, N, O (A, B, C, D) | water | water | 18.0 | 1783 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 337 (UniProt: Q88K39)
| PDB | External Database | Details |
|---|---|---|
| Met 173 | Lys 198 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 144792.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 774.7 | |
| All* | Total formula weight | 145566.8 |
