6WXA

Crystal structure of truncated Streptococcal bacteriophage hyaluronidase complexed with unsaturated hyaluronan hexa-saccharides

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Hyaluronan lyase	polymer	293	31532.4	3	UniProt (P15316) Pfam (PF07212) In PDB	Streptococcus pyogenes phage H4489A	HylP, Hyaluronate Lyase
2	D, G	4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1138.0	2	In PDB GlyTouCan (G36225QF)
3	E	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		979.8	1	In PDB GlyTouCan (G35556TW)
4	F, H	4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		758.6	2	In PDB GlyTouCan (G58744KX)
5	B	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
6		water	water		18.0	138	Chemie (HOH)

Sequence modifications

A, B, C: 87 - 371 (UniProt: P15316)

PDB	External Database	Details
Leu 372	-	expression tag
Glu 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94597.3
Branched	Number of molecules	5
	Total formula weight	4773.0
Non-Polymers*	Number of molecules	1
	Total formula weight	58.7
All*	Total formula weight	99429.0

*Water molecules are not included.