6WW9

Crystal structure of human REV7(R124A)-SHLD3(35-58) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Mitotic spindle assembly checkpoint protein MAD2B	polymer	211	24237.2	2	UniProt (Q9UI95) Pfam (PF02301) In PDB	Homo sapiens (Human)	Mitotic arrest deficient 2-like protein 2,MAD2-like protein 2,REV7 homolog,hREV7
2	X, Y	Shieldin complex subunit 3	polymer	35	4094.8	2	UniProt (Q6ZNX1) In PDB	Homo sapiens (Human)	REV7-interacting novel NHEJ regulator 1,Shield complex subunit 3

Sequence modifications

A, B: 2 - 211 (UniProt: Q9UI95)

PDB	External Database	Details
Ser 1	-	expression tag
Ala 124	Arg 124	engineered mutation

X, Y: 35 - 58 (UniProt: Q6ZNX1)

PDB	External Database	Details
Met 34	-	initiating methionine
Ala 59	-	expression tag
Ser 60	-	expression tag
Arg 61	-	expression tag
Glu 62	-	expression tag
Glu 63	-	expression tag
Ile 64	-	expression tag
Met 65	-	expression tag
Ala 66	-	expression tag
Thr 67	-	expression tag
Leu 68	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	56664.2
All*	Total formula weight	56664.2

*Water molecules are not included.