6WVJ

Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	314	34842.4	2	UniProt (P20429) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Bacillus subtilis (strain 168)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1193	133847.9	1	UniProt (A0A2J0WBQ0) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P37870) In PDB	Bacillus subtilis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1199	134445.0	1	UniProt (A0A063XB23) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P37871) In PDB	Bacillus subtilis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	F	DNA-directed RNA polymerase subunit omega	polymer	67	7766.9	1	UniProt (A0A063XI46) Pfam (PF01192) UniProt (by SIFTS) (O35011) In PDB	Bacillus subtilis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	T	DNA (5'-D(*TP*GP*TP*CP*GP*GP*GP*CP*GP*TP*CP*CP*GP*CP*GP*CP*GP*CP*C)-3')	polymer	19	5814.7	1		Escherichia coli BL21(DE3)
6	R	RNA (5'-R(P*GP*GP*CP*GP*CP*GP*CP*G)-3')	polymer	8	2596.6	1		Escherichia coli BL21(DE3)
7	N	DNA (5'-D(P*AP*CP*GP*CP*CP*CP*GP*AP*CP*A)-3')	polymer	10	2999.0	1		Escherichia coli BL21(DE3)
8	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	357154.9
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	357310.0

*Water molecules are not included.