6WSG

ClpX-ClpP complex bound to ssrA-tagged GFP, intermediate complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	ATP-dependent Clp protease ATP-binding subunit ClpX	polymer	388	42355.8	6	UniProt (P0A6H1) Pfam (PF07724) Pfam (PF10431)	Escherichia coli	ATP-dependent unfoldase ClpX
2	G, H, I, J, K... (L, K, J, I, H...)	ATP-dependent Clp protease proteolytic subunit	polymer	207	23468.9	7	UniProt (P0A6G7) Pfam (PF00574)	Escherichia coli	Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti
3	N (S)	Green fluorescent protein, + ssrA tag	polymer	274	30341.7	1	UniProt (P42212)	Aequorea victoria (Jellyfish)
4	O, Q, S, U, W (B, C, D, E, F)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	5	Chemie (AGS)
5	P, R, T, V (B, C, D, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	AA, BA, CA, DA, X... (I, H, N, M, L...)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 62 - 424 (UniProt: P0A6H1)

PDB	External Database	Details
Met 37	-	expression tag
Gly 38	-	expression tag
Ser 39	-	expression tag
Ser 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
Asp 47	-	expression tag
Tyr 48	-	expression tag
Asp 49	-	expression tag
Ile 50	-	expression tag
Pro 51	-	expression tag
Thr 52	-	expression tag
Thr 53	-	expression tag
Glu 54	-	expression tag
Asn 55	-	expression tag
Leu 56	-	expression tag
Tyr 57	-	expression tag
Phe 58	-	expression tag
Gln 59	-	expression tag
Gly 60	-	expression tag
Ser 61	-	expression tag
Ser 169	Cys 169	conflict
Glu 408	Lys 408	conflict

L, K, J, I, H, N, M: 2 - 193 (UniProt: P0A6G7)

PDB	External Database	Details
Glu 194	-	expression tag
Asn 195	-	expression tag
Leu 196	-	expression tag
Tyr 197	-	expression tag
Phe 198	-	expression tag
Gln 199	-	expression tag
Ser 200	-	expression tag
Leu 201	-	expression tag
Glu 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

S: 32 - 258 (UniProt: P42212)

PDB	External Database	Details
Met 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Asp 16	-	expression tag
Tyr 17	-	expression tag
Asp 18	-	expression tag
Ile 19	-	expression tag
Pro 20	-	expression tag
Thr 21	-	expression tag
Thr 22	-	expression tag
Glu 23	-	expression tag
Asn 24	-	expression tag
Leu 25	-	expression tag
Tyr 26	-	expression tag
Phe 27	-	expression tag
Gln 28	-	expression tag
Gly 29	-	expression tag
Ser 30	-	expression tag
Arg 31	-	expression tag
Gly 94	Ser 65	conflict
Ala 101	Ser 72	conflict

S: 259 - 279 (PDB: 6WSG)

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	448758.7
Non-Polymers*	Number of molecules	9
	Total formula weight	2713.5
All*	Total formula weight	451472.2

*Water molecules are not included.