6WRF

ClpX-ClpP complex bound to GFP-ssrA, recognition complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (B, C, D, E, F...)	ATP-dependent Clp protease ATP-binding subunit ClpX	polymer	388	42355.8	6	UniProt (P0A6H1) Pfam (PF07724) Pfam (PF10431)	Escherichia coli (strain K12)	ATP-dependent unfoldase ClpX
2	F, G, H, I, J... (H, I, J, K, L...)	ATP-dependent Clp protease proteolytic subunit	polymer	207	23468.9	7	UniProt (P0A6G7) Pfam (PF00574)	Escherichia coli (strain K12)	Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti
3	M (S)	Green fluorescent protein	polymer	274	30341.7	1	UniProt (P42212)	Aequorea victoria (Jellyfish)
4	O, Q, S, U, X (B, C, D, E, A)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	5	Chemie (AGS)
5	P, R, T, V, Y (B, C, D, E, A)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
6	W (F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7	AA, BA, CA, DA, EA... (I, J, K, L, M...)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

B, C, D, E, F, A: 62 - 424 (UniProt: P0A6H1)

PDB	External Database	Details
Met 37	-	initiating methionine
Gly 38	-	expression tag
Ser 39	-	expression tag
Ser 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
Asp 47	-	expression tag
Tyr 48	-	expression tag
Asp 49	-	expression tag
Ile 50	-	expression tag
Pro 51	-	expression tag
Thr 52	-	expression tag
Thr 53	-	expression tag
Glu 54	-	expression tag
Asn 55	-	expression tag
Leu 56	-	expression tag
Tyr 57	-	expression tag
Phe 58	-	expression tag
Gln 59	-	expression tag
Gly 60	-	expression tag
Ser 61	-	expression tag
Ser 169	Cys 169	conflict
Glu 408	Lys 408	conflict

H, I, J, K, L, M, N: 2 - 193 (UniProt: P0A6G7)

PDB	External Database	Details
Glu 194	-	expression tag
Asn 195	-	expression tag
Leu 196	-	expression tag
Tyr 197	-	expression tag
Phe 198	-	expression tag
Gln 199	-	expression tag
Ser 200	-	expression tag
Leu 201	-	expression tag
Glu 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

S: 27 - 253 (UniProt: P42212)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Tyr 12	-	expression tag
Asp 13	-	expression tag
Ile 14	-	expression tag
Pro 15	-	expression tag
Thr 16	-	expression tag
Thr 17	-	expression tag
Glu 18	-	expression tag
Asn 19	-	expression tag
Leu 20	-	expression tag
Tyr 21	-	expression tag
Phe 22	-	expression tag
Gln 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
Arg 26	-	expression tag
Gly 89	Ser 65	conflict
Ala 96	Ser 72	conflict
Gly 254	-	expression tag
Gly 255	-	expression tag
Gly 256	-	expression tag
Tyr 257	-	expression tag
Gly 258	-	expression tag
Gly 259	-	expression tag
Gly 260	-	expression tag
Gly 261	-	expression tag
Gly 262	-	expression tag
Gly 263	-	expression tag
Gly 264	-	expression tag
Gly 265	-	expression tag
Gly 266	-	expression tag
Ser 267	-	expression tag
Glu 268	-	expression tag
Asn 269	-	expression tag
Tyr 270	-	expression tag
Ala 271	-	expression tag
Leu 272	-	expression tag
Ala 273	-	expression tag
Ala 274	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	448758.7
Non-Polymers*	Number of molecules	11
	Total formula weight	3165.0
All*	Total formula weight	451923.7

*Water molecules are not included.