6WP3

Pyruvate Kinase M2 Mutant-K433Q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Pyruvate kinase PKM	polymer	550	60049.0	2	UniProt (P14618) Pfam (PF00224) Pfam (PF02887)	Homo sapiens (Human)	Cytosolic thyroid hormone-binding protein,CTHBP,Opa-interacting protein 3,OIP-3,Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,THBP1,Tumor M2-PK,p58
2	C, D, E, F, J... (A, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
3	G, M (A, B)	OXALATE ION	non-polymer		88.0	2	Chemie (OXL)
4	H, N (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, O (A, B)	NITRATE ION	non-polymer		62.0	2	Chemie (NO3)
6	P (B)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
7	Q (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	R, S (A, B)	water	water		18.0	385	Chemie (HOH)

Sequence modifications

A, B: 1 - 531 (UniProt: P14618)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gln 433	Lys 433	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120098.1
Non-Polymers*	Number of molecules	15
	Total formula weight	1067.9
All*	Total formula weight	121166.0

*Water molecules are not included.