6WMU

E. coli RNAPs70-SspA-gadA DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1430	158314.9	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor RpoD	polymer	613	70352.2	1	UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) In PDB	Escherichia coli	Sigma-70
6	G	DNA NT-strand	polymer	37	11238.2	1		Escherichia coli
7	H	DNA T-strand	polymer	27	8389.4	1		Escherichia coli
8	I	DNA NT-strand downstream	polymer	11	3350.2	1		Escherichia coli
9	J	DNA T-strand downstream	polymer	11	3359.2	1		Escherichia coli
10	K, L	Stringent starvation protein A	polymer	232	26504.3	2	UniProt (A0A1X3LEF3) Pfam (PF02798) Pfam (PF00043) UniProt (by SIFTS) (P0ACA3) In PDB	Escherichia coli TA054
11	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
12	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

D: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Arg 1410	-	expression tag
Arg 1411	-	expression tag
Ala 1412	-	expression tag
Ser 1413	-	expression tag
Glu 1414	-	expression tag
Asn 1415	-	expression tag
Leu 1416	-	expression tag
Tyr 1417	-	expression tag
Phe 1418	-	expression tag
Gln 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

K, L: 1 - 212 (UniProt: A0A1X3LEF3)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	542200.5
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	542355.6

*Water molecules are not included.