6WHM

The crystal structure of the 2009/H1N1/California PA endonuclease wild type bound to DNA oligomer TAGC (cleaved TTAGCATT, 5mM overnight DNA soak)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	PA-X protein,Polymerase acidic protein	polymer	197	23148.3	1	UniProt (A0A481PFF5) UniProt (C6H0Y9) Pfam (PF00603)	Influenza A virus
2	B (B)	DNA (5'-D(P*TP*AP*GP*C)-3')	polymer	8	2416.6	1		synthetic construct
3	C, D (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E, I (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
6	G, H (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	J, K (A, B)	water	water		18.0	136	Chemie (HOH)

Sequence modifications

A: 1 - 50 (UniProt: A0A481PFF5)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 51	-	linker
Gly 52	-	linker
Ser 53	-	linker
Lys 73	-	linker

A: 74 - 196 (UniProt: C6H0Y9)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25565.0
Non-Polymers*	Number of molecules	7
	Total formula weight	479.9
All*	Total formula weight	26044.8

*Water molecules are not included.