6WHI

Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated protein Csy1	polymer	434	49194.2	1	UniProt (Q02ML9) Pfam (PF09611)	Pseudomonas aeruginosa	Csy1 (Cas8f)
2	B (B)	Type I-F CRISPR-associated protein Csy2	polymer	327	36244.1	1	UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0)	Pseudomonas aeruginosa	Csy2 (Cas5f)
3	C, D, E, F, G... (C, D, E, F, G...)	CRISPR-associated protein Csy3	polymer	360	39778.6	6	UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1)	Pseudomonas aeruginosa	Csy3 (Cas7f)
4	I (M)	RNA (60-MER)	polymer	60	19265.4	1	GenBank (313291946)	Pseudomonas aeruginosa
5	J (L)	CRISPR-associated endonuclease Cas6/Csy4	polymer	187	21427.5	1	UniProt (Q02MM2) Pfam (PF09618)	Pseudomonas aeruginosa	Csy4 (Cas6f)
6	K, L (I, J)	anti-CRISPR AcrIF9	polymer	68	7863.8	2	UniProt (C0AVY5)	Proteus penneri
7	M, N (N, Q)	non-target dsDNA	polymer	77	23797.2	2		Pseudomonas aeruginosa
8	O, P (P, O)	non-target dsDNA	polymer	77	23687.2	2		Pseudomonas aeruginosa

Sequence modifications

C, D, E, F, G, H: 21 - 361 (UniProt: A0A444M080)

PDB	External Database	Details
Met 2	-	expression tag
Lys 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Glu 12	-	expression tag
Asn 13	-	expression tag
Leu 14	-	expression tag
Tyr 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Ser 18	-	expression tag
Asn 19	-	expression tag
Ala 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	475499.3
All*	Total formula weight	475499.3

*Water molecules are not included.