6WHI
Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated protein Csy1 | polymer | 434 | 49194.2 | 1 | UniProt (Q02ML9) Pfam (PF09611) In PDB | Pseudomonas aeruginosa | Csy1 (Cas8f) |
2 | B | Type I-F CRISPR-associated protein Csy2 | polymer | 327 | 36244.1 | 1 | UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB | Pseudomonas aeruginosa | Csy2 (Cas5f) |
3 | C, D, E, F, G... | CRISPR-associated protein Csy3 | polymer | 360 | 39778.6 | 6 | UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB | Pseudomonas aeruginosa | Csy3 (Cas7f) |
4 | M | RNA (60-MER) | polymer | 60 | 19265.4 | 1 | GenBank (313291946) | Pseudomonas aeruginosa | |
5 | L | CRISPR-associated endonuclease Cas6/Csy4 | polymer | 187 | 21427.5 | 1 | UniProt (Q02MM2) Pfam (PF09618) In PDB | Pseudomonas aeruginosa | Csy4 (Cas6f) |
6 | I, J | anti-CRISPR AcrIF9 | polymer | 68 | 7863.8 | 2 | UniProt (C0AVY5) In PDB | Proteus penneri | |
7 | N, Q | non-target dsDNA | polymer | 77 | 23797.2 | 2 | Pseudomonas aeruginosa | ||
8 | P, O | non-target dsDNA | polymer | 77 | 23687.2 | 2 | Pseudomonas aeruginosa |
Sequence modifications
C, D, E, F, G, H: 21 - 361 (UniProt: A0A444M080)
PDB | External Database | Details |
---|---|---|
Met 2 | - | expression tag |
Lys 3 | - | expression tag |
Ser 4 | - | expression tag |
Ser 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
His 11 | - | expression tag |
Glu 12 | - | expression tag |
Asn 13 | - | expression tag |
Leu 14 | - | expression tag |
Tyr 15 | - | expression tag |
Phe 16 | - | expression tag |
Gln 17 | - | expression tag |
Ser 18 | - | expression tag |
Asn 19 | - | expression tag |
Ala 20 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 16 |
Total formula weight | 475499.3 | |
All* | Total formula weight | 475499.3 |