6WG3

Cryo-EM structure of human Cohesin-NIPBL-DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Structural maintenance of chromosomes protein 1A	polymer	1233	143484.1	1	UniProt (Q14683) Pfam (PF02463) Pfam (PF06470) In PDB	Homo sapiens (Human)	SMC-1A,Sb1.8
2	B	Structural maintenance of chromosomes protein 3	polymer	1217	141770.6	1	UniProt (Q9UQE7) Pfam (PF02463) Pfam (PF06470) In PDB	Homo sapiens (Human)	SMC-3,Basement membrane-associated chondroitin proteoglycan,Bamacan,Chondroitin sulfate proteoglycan 6,Chromosome-associated polypeptide,hCAP
3	C	Double-strand-break repair protein rad21 homolog	polymer	631	71556.1	1	UniProt (O60216) Pfam (PF04825) Pfam (PF04824) In PDB	Homo sapiens (Human)	hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog
4	D	Cohesin subunit SA-1	polymer	1272	146075.7	1	UniProt (Q8WVM7) Pfam (PF08514) Pfam (PF21581) In PDB	Homo sapiens (Human)	SCC3 homolog 1,Stromal antigen 1
5	E	Nipped-B-like protein	polymer	1652	188151.7	1	UniProt (Q6KC79) Pfam (PF12830) Pfam (PF12765) In PDB	Homo sapiens (Human)	Delangin,SCC2 homolog
6	F	DNA (51-MER)	polymer	51	15928.6	1		Homo sapiens
7	G	DNA (51-MER)	polymer	51	15468.9	1		Homo sapiens
8	A, B	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)

Sequence modifications

A: 1 - 1233 (UniProt: Q14683)

PDB	External Database	Details
Gln 1157	Glu 1157	engineered mutation

B: 1 - 1217 (UniProt: Q9UQE7)

PDB	External Database	Details
Gln 1144	Glu 1144	engineered mutation

C: 1 - 631 (UniProt: O60216)

PDB	External Database	Details
Ala 172	Arg 172	engineered mutation
Ala 279	Asp 279	engineered mutation
Ala 450	Arg 450	engineered mutation

D: 1 - 1258 (UniProt: Q8WVM7)

PDB	External Database	Details
Gly 1259	-	expression tag
Ala 1260	-	expression tag
Pro 1261	-	expression tag
Met 1262	-	expression tag
Arg 1263	-	expression tag
Ser 1264	-	expression tag
Gly 1265	-	expression tag
Ala 1266	-	expression tag
Leu 1267	-	expression tag
Glu 1268	-	expression tag
Val 1269	-	expression tag
Leu 1270	-	expression tag
Phe 1271	-	expression tag
Gln 1272	-	expression tag

E: 1163 - 2804 (UniProt: Q6KC79)

PDB	External Database	Details
Met 1153	-	expression tag
Ser 1154	-	expression tag
Tyr 1155	-	expression tag
Tyr 1156	-	expression tag
His 1157	-	expression tag
His 1158	-	expression tag
His 1159	-	expression tag
His 1160	-	expression tag
His 1161	-	expression tag
His 1162	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	722435.6
Non-Polymers*	Number of molecules	2
	Total formula weight	1012.4
All*	Total formula weight	723448.0

*Water molecules are not included.