6WG3
Cryo-EM structure of human Cohesin-NIPBL-DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Structural maintenance of chromosomes protein 1A | polymer | 1233 | 143484.1 | 1 | UniProt (Q14683) Pfam (PF02463) Pfam (PF06470) | Homo sapiens (Human) | SMC-1A,Sb1.8 |
| 2 | B (B) | Structural maintenance of chromosomes protein 3 | polymer | 1217 | 141770.6 | 1 | UniProt (Q9UQE7) Pfam (PF02463) Pfam (PF06470) | Homo sapiens (Human) | SMC-3,Basement membrane-associated chondroitin proteoglycan,Bamacan,Chondroitin sulfate proteoglycan 6,Chromosome-associated polypeptide,hCAP |
| 3 | C (C) | Double-strand-break repair protein rad21 homolog | polymer | 631 | 71556.1 | 1 | UniProt (O60216) Pfam (PF04825) Pfam (PF04824) | Homo sapiens (Human) | hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog |
| 4 | D (D) | Cohesin subunit SA-1 | polymer | 1272 | 146075.7 | 1 | UniProt (Q8WVM7) Pfam (PF08514) Pfam (PF21581) Pfam (PF24571) | Homo sapiens (Human) | SCC3 homolog 1,Stromal antigen 1 |
| 5 | E (E) | Nipped-B-like protein | polymer | 1652 | 188151.7 | 1 | UniProt (Q6KC79) Pfam (PF12765) Pfam (PF12830) | Homo sapiens (Human) | Delangin,SCC2 homolog |
| 6 | F (F) | DNA (51-MER) | polymer | 51 | 15928.6 | 1 | Homo sapiens | ||
| 7 | G (G) | DNA (51-MER) | polymer | 51 | 15468.9 | 1 | Homo sapiens | ||
| 8 | H, I (A, B) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) |
Sequence modifications
A: 1 - 1233 (UniProt: Q14683)
B: 1 - 1217 (UniProt: Q9UQE7)
C: 1 - 631 (UniProt: O60216)
D: 1 - 1258 (UniProt: Q8WVM7)
E: 1163 - 2804 (UniProt: Q6KC79)
| PDB | External Database | Details |
|---|---|---|
| Gln 1157 | Glu 1157 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gln 1144 | Glu 1144 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 172 | Arg 172 | engineered mutation |
| Ala 279 | Asp 279 | engineered mutation |
| Ala 450 | Arg 450 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 1259 | - | expression tag |
| Ala 1260 | - | expression tag |
| Pro 1261 | - | expression tag |
| Met 1262 | - | expression tag |
| Arg 1263 | - | expression tag |
| Ser 1264 | - | expression tag |
| Gly 1265 | - | expression tag |
| Ala 1266 | - | expression tag |
| Leu 1267 | - | expression tag |
| Glu 1268 | - | expression tag |
| Val 1269 | - | expression tag |
| Leu 1270 | - | expression tag |
| Phe 1271 | - | expression tag |
| Gln 1272 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1153 | - | expression tag |
| Ser 1154 | - | expression tag |
| Tyr 1155 | - | expression tag |
| Tyr 1156 | - | expression tag |
| His 1157 | - | expression tag |
| His 1158 | - | expression tag |
| His 1159 | - | expression tag |
| His 1160 | - | expression tag |
| His 1161 | - | expression tag |
| His 1162 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 722435.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1012.4 | |
| All* | Total formula weight | 723448.0 |
