6WBX

Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S Class 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DUF3367 domain-containing protein	polymer	1438	152818.6	1	UniProt (A0A418KZ72) Pfam (PF11847)	Mycobacteroides abscessus
2	B, C (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

A: 1 - 1410 (UniProt: A0A418KZ72)

PDB	External Database	Details
Met -27	-	initiating methionine
Asp -26	-	expression tag
Tyr -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Lys -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Tyr -1	-	expression tag
Val 0	-	expression tag
Leu 87	Met 87	conflict
Ile 1078	Val 1078	conflict
Asp 1083	Asn 1083	conflict
Asn 1105	Asp 1105	conflict
Ala 1172	Thr 1172	conflict
Cys 1293	Arg 1293	conflict
Ser 1389	Arg 1389	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	152818.6
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	152898.8

*Water molecules are not included.