6WB7

Acarbose Kinase AcbK as a Complex with Acarbose and AMP-PNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Acarbose 7(IV)-phosphotransferase	polymer	319	33686.8	4	UniProt (Q8RMD4) Pfam (PF00294) In PDB	Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110)	Acarbose 7-kinase
2	E, F, G, H	4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		645.6	4	In PDB BIRD (PRD_900007) GlyTouCan (G66431MI)		alpha-acarbose
3	A, B, C, D	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)
4	A, B, C, D	MANGANESE (II) ION	non-polymer		54.9	5	Chemie (MN)
5	A, B, C, D	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6		water	water		18.0	380	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 299 (UniProt: Q8RMD4)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134747.2
Branched	Number of molecules	4
	Total formula weight	2582.4
Non-Polymers*	Number of molecules	13
	Total formula weight	2391.4
All*	Total formula weight	139721.0

*Water molecules are not included.