6W7Z

RNF12 RING domain in complex with Ube2d2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17188.6	1	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B	E3 ubiquitin-protein ligase RLIM	polymer	99	11142.5	1	UniProt (Q9NVW2) Pfam (PF13639) In PDB	Homo sapiens (Human)	LIM domain-interacting RING finger protein,RING finger LIM domain-binding protein,R-LIM,RING finger protein 12,RING-type E3 ubiquitin transferase RLIM,Renal carcinoma antigen NY-REN-43, RNF12-RING
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5		water	water		18.0	146	Chemie (HOH)

Sequence modifications

A: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

B: 530 - 624 (UniProt: Q9NVW2)

PDB	External Database	Details
Gly 526	-	expression tag
Pro 527	-	expression tag
Leu 528	-	expression tag
Gly 529	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	28331.1
Non-Polymers*	Number of molecules	3
	Total formula weight	222.9
All*	Total formula weight	28554.0

*Water molecules are not included.