6W4F

NMR-driven structure of KRAS4B-GDP homodimer on a lipid bilayer nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	Apolipoprotein A-I	polymer	200	23234.3	2	UniProt (P02647) Pfam (PF01442)	Homo sapiens (Human)	ApoA-I,Apolipoprotein A1
2	C, D (B, C)	GTPase KRas	polymer	184	21043.0	2	UniProt (P01116) Pfam (PF00071)	Homo sapiens (Human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
3	AA, AC, BA, BC, CA... (D, C, A, B)	1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		787.1	64	Chemie (PCW)
4	AB, BB, CB, DB, EB... (D, C, A, B)	O-[(S)-({(2R)-2,3-bis[(9Z)-octadec-9-enoyloxy]propyl}oxy)(hydroxy)phosphoryl]-L-serine	non-polymer		788.0	16	Chemie (17F) PubChem (16219821) PubChem (58556791) PubChem (57369815) PubChem (125750) PubChem (3451126) PubChem (5830256) PubChem (6438639) PubChem (124226306) PubChem (145954209) PubChem (15409351) PubChem (99650225) PubChem (122607770) PubChem (124629033) PubChem (9547077) PubChem (9547078) PubChem (9898005)
5	HB, YB (B, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
6	IB, ZB (B, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)

Sequence modifications

A, D: 201 - 398 (UniProt: P02647)

PDB	External Database	Details
Gly 199	-	expression tag
Pro 200	-	expression tag

B, C: 2 - 185 (UniProt: P01116)

PDB	External Database	Details
Asp 12	Gly 12	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88554.5
Non-Polymers*	Number of molecules	84
	Total formula weight	63919.4
All*	Total formula weight	152474.0

*Water molecules are not included.