6W1X
Cryo-EM structure of anti-CRISPR AcrIF9, bound to the type I-F crRNA-guided CRISPR surveillance complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated protein Csy1 | polymer | 434 | 49194.2 | 1 | UniProt (Q02ML9) | Pseudomonas aeruginosa | Csy1 (Cas8f) |
| 2 | B (B) | Type I-F CRISPR-associated protein Csy2 | polymer | 327 | 36244.1 | 1 | UniProt (B3G161) UniProt (by SIFTS) (Q02MM0) | Pseudomonas aeruginosa | Csy2 (Cas5f) |
| 3 | C, D, E, F, G... (C, D, E, F, G...) | CRISPR-associated protein Csy3 | polymer | 360 | 39778.6 | 6 | UniProt (A0A444M080) UniProt (by SIFTS) (Q02MM1) | Pseudomonas aeruginosa | Csy3 (Cas7f) |
| 4 | I (M) | RNA (60-MER) | polymer | 60 | 19265.4 | 1 | GenBank (313291946) | Pseudomonas aeruginosa | |
| 5 | J (L) | CRISPR-associated endonuclease Cas6/Csy4 | polymer | 187 | 21427.5 | 1 | UniProt (Q02MM2) | Pseudomonas aeruginosa | Csy4 (Cas6f) |
| 6 | K, L (I, J) | anti-CRISPR AcrIF9 | polymer | 68 | 7863.8 | 2 | UniProt (C0AVY5) | Proteus penneri |
Sequence modifications
C, D, E, F, G, H: 21 - 361 (UniProt: A0A444M080)
| PDB | External Database | Details |
|---|---|---|
| Met 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Ser 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| Glu 12 | - | expression tag |
| Asn 13 | - | expression tag |
| Leu 14 | - | expression tag |
| Tyr 15 | - | expression tag |
| Phe 16 | - | expression tag |
| Gln 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Asn 19 | - | expression tag |
| Ala 20 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 380530.4 | |
| All* | Total formula weight | 380530.4 |
