6VW0
Mycobacterium tuberculosis RNAP S456L mutant open promoter complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 347 | 37745.3 | 2 | UniProt (A5U8D3) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P9WGZ1) In PDB | Mycobacterium tuberculosis | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1179 | 130096.9 | 1 | UniProt (V9Z879) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P9WGY9) In PDB | Mycobacterium tuberculosis | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1326 | 148202.2 | 1 | UniProt (A5U053) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P9WGY7) In PDB | Mycobacterium tuberculosis | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | E | DNA-directed RNA polymerase subunit omega | polymer | 110 | 11777.0 | 1 | UniProt (A0A0T9N9K3) Pfam (PF01192) UniProt (by SIFTS) (P9WGY5) In PDB | Mycobacterium tuberculosis | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | F | RNA polymerase sigma factor SigA | polymer | 531 | 58169.5 | 1 | UniProt (P9WGI0) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P9WGI1) In PDB | Mycobacterium tuberculosis | Sigma-A |
6 | J | RNA polymerase-binding protein RbpA | polymer | 111 | 12993.7 | 1 | UniProt (P9WHJ4) Pfam (PF13397) UniProt (by SIFTS) (P9WHJ5) In PDB | Mycobacterium tuberculosis | |
7 | M | RNA polymerase-binding transcription factor CarD | polymer | 162 | 17933.4 | 1 | UniProt (P9WJG2) Pfam (PF02559) Pfam (PF21095) UniProt (by SIFTS) (P9WJG3) In PDB | Mycobacterium tuberculosis | |
8 | O | DNA (65-MER) | polymer | 90 | 27907.8 | 1 | Mycobacterium tuberculosis | ||
9 | P | DNA (65-MER) | polymer | 90 | 27618.6 | 1 | Mycobacterium tuberculosis | ||
10 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
11 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
C: 7 - 1178 (UniProt: V9Z879)
D: 1 - 1316 (UniProt: A5U053)
E: 2 - 110 (UniProt: A0A0T9N9K3)
F: 1 - 528 (UniProt: P9WGI0)
PDB | External Database | Details |
---|---|---|
Leu 456 | Ser 450 | engineered mutation |
Leu 1179 | - | expression tag |
Ala 1180 | - | expression tag |
Arg 1181 | - | expression tag |
His 1182 | - | expression tag |
Gly 1183 | - | expression tag |
Gly 1184 | - | expression tag |
Ser 1185 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ala 0 | - | expression tag |
His 1317 | - | expression tag |
His 1318 | - | expression tag |
His 1319 | - | expression tag |
His 1320 | - | expression tag |
His 1321 | - | expression tag |
His 1322 | - | expression tag |
His 1323 | - | expression tag |
His 1324 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -2 | - | expression tag |
Pro -1 | - | expression tag |
His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 10 |
Total formula weight | 510189.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 510344.8 |