6VVT
Crystal structure of a Mycobacterium smegmatis transcription initiation complex with Rifampicin-resistant RNA polymerase and antibiotic Sorangicin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | J | RNA polymerase-binding protein RbpA | polymer | 114 | 13078.7 | 1 | UniProt (A0QZ11) Pfam (PF13397) In PDB | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) | |
| 2 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 350 | 37959.4 | 2 | UniProt (A0QSL8) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 3 | C | DNA-directed RNA polymerase subunit beta | polymer | 1169 | 128706.2 | 1 | UniProt (P60281) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 4 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1317 | 146712.9 | 1 | UniProt (A0QS66) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 5 | E | DNA-directed RNA polymerase subunit omega | polymer | 107 | 11544.8 | 1 | UniProt (A0QWT1) Pfam (PF01192) In PDB | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 6 | F | RNA polymerase sigma factor SigA | polymer | 466 | 51573.6 | 1 | UniProt (A0QW02) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) | |
| 7 | O | DNA (31-MER) | polymer | 31 | 9565.2 | 1 | synthetic construct | ||
| 8 | P | DNA (26-MER) | polymer | 26 | 7930.2 | 1 | synthetic construct | ||
| 9 | C | SORANGICIN A | non-polymer | 807.0 | 1 | Chemie (SRN) | |||
| 10 | C | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 11 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 12 | D, F | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
| 13 | water | water | 18.0 | 15 | Chemie (HOH) |
Sequence modifications
C: 1 - 1169 (UniProt: P60281)
| PDB | External Database | Details |
|---|---|---|
| Leu 447 | Ser 447 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 445030.4 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1864.5 | |
| All* | Total formula weight | 446894.9 |
