6VU3
Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (0) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (D7ZEN7) UniProt (by SIFTS) (P0AG48) Pfam (PF00829) | Escherichia coli | |
| 2 | B (1) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (D7Z9G0) UniProt (by SIFTS) (P61175) Pfam (PF00237) | Escherichia coli | |
| 3 | C (2) | 50S ribosomal protein L23 | polymer | 94 | 10661.6 | 1 | UniProt (S1EUY1) UniProt (by SIFTS) (P0ADZ0) Pfam (PF00276) | Escherichia coli | |
| 4 | D (3) | 50S ribosomal protein L24 | polymer | 103 | 11208.1 | 1 | UniProt (F4SQ36) UniProt (by SIFTS) (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli | |
| 5 | E (4) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (D7XH79) UniProt (by SIFTS) (P68919) Pfam (PF01386) | Escherichia coli | |
| 6 | F (5) | NT DNA | polymer | 36 | 11042.1 | 1 | synthetic construct | ||
| 7 | G (6) | T DNA | polymer | 27 | 8155.2 | 1 | synthetic construct | ||
| 8 | H (7) | mRNA with 12 nt long spacer | polymer | 29 | 9238.3 | 1 | synthetic construct | ||
| 9 | I (9) | 50S ribosomal protein L10 | polymer | 148 | 15981.5 | 1 | UniProt (A0A3W3FJZ4) UniProt (by SIFTS) (P0A7J3) Pfam (PF00466) | Escherichia coli | |
| 10 | J, P (A, B) | E-site and A-site tRNA (fMet) | polymer | 76 | 24496.6 | 2 | GenBank (1870104878) | Escherichia coli | |
| 11 | K (AA) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) UniProt (by SIFTS) (P0A8V2) Pfam (PF04561) Pfam (PF04563) Pfam (PF10385) Pfam (PF04565) Pfam (PF00562) Pfam (PF04560) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 12 | L (AB) | Transcription termination/antitermination protein NusG | polymer | 181 | 20560.5 | 1 | UniProt (U9XYQ6) UniProt (by SIFTS) (P0AFG0) Pfam (PF02357) Pfam (PF00467) | Escherichia coli | |
| 13 | M, N (AC, AD) | DNA-directed RNA polymerase subunit alpha | polymer | 230 | 25497.0 | 2 | UniProt (A0A5F0PYH4) UniProt (by SIFTS) (P0A7Z4) Pfam (PF01193) Pfam (PF01000) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 14 | O (AE) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) UniProt (by SIFTS) (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 15 | Q (C) | 30S ribosomal protein S18 | polymer | 66 | 7734.9 | 1 | UniProt (I2SQR9) UniProt (by SIFTS) (P0A7T7) Pfam (PF01084) | Escherichia coli | |
| 16 | R (D) | 16S rRNA | polymer | 1542 | 499690.0 | 1 | GenBank (1732209707) | Escherichia coli | |
| 17 | S (E) | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (I4T5W9) UniProt (by SIFTS) (P0A7U7) Pfam (PF01649) | Escherichia coli | |
| 18 | T (F) | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (E9TH65) UniProt (by SIFTS) (P68679) Pfam (PF01165) | Escherichia coli | |
| 19 | U (G) | 30S ribosomal protein S2 | polymer | 225 | 25072.9 | 1 | UniProt (C3TPN2) UniProt (by SIFTS) (P0A7V0) Pfam (PF00318) | Escherichia coli | |
| 20 | V (H) | 30S ribosomal protein S1 | polymer | 557 | 61238.9 | 1 | UniProt (D6HUW2) UniProt (by SIFTS) (P0AG67) Pfam (PF00575) | Escherichia coli | |
| 21 | W (I) | 30S ribosomal protein S3 | polymer | 208 | 23249.0 | 1 | UniProt (A0A376HTV6) UniProt (by SIFTS) (P0A7V3) Pfam (PF07650) Pfam (PF00189) | Escherichia coli | |
| 22 | X (J) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (L3PZ69) UniProt (by SIFTS) (P0A7V8) Pfam (PF00163) Pfam (PF01479) | Escherichia coli | |
| 23 | Y (K) | 30S ribosomal protein S5 | polymer | 156 | 16475.0 | 1 | UniProt (T9SGC3) UniProt (by SIFTS) (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli | |
| 24 | Z (L) | 30S ribosomal protein S6 | polymer | 104 | 12126.0 | 1 | UniProt (A0A376ZL25) UniProt (by SIFTS) (P02358) Pfam (PF01250) | Escherichia coli | |
| 25 | AA (M) | 30S ribosomal protein S7 | polymer | 151 | 16861.5 | 1 | UniProt (S1D5F0) UniProt (by SIFTS) (P02359) Pfam (PF00177) | Escherichia coli | |
| 26 | BA (N) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (D8A1L7) UniProt (by SIFTS) (P0A7W7) Pfam (PF00410) | Escherichia coli | |
| 27 | CA (O) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (T9TH92) UniProt (by SIFTS) (P0A7X3) Pfam (PF00380) | Escherichia coli | |
| 28 | DA (P) | 30S ribosomal protein S10 | polymer | 99 | 11254.0 | 1 | UniProt (A0A073G203) UniProt (by SIFTS) (P0A7R5) Pfam (PF00338) | Escherichia coli | |
| 29 | EA (Q) | 30S ribosomal protein S11 | polymer | 117 | 12487.2 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli | Small ribosomal subunit protein uS11 |
| 30 | FA (R) | 30S ribosomal protein S12 | polymer | 124 | 13768.2 | 1 | UniProt (D7ZAN0) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3) | Escherichia coli | |
| 31 | GA (S) | 30S ribosomal protein S14 | polymer | 100 | 11475.4 | 1 | UniProt (A0A1X3K3H0) UniProt (by SIFTS) (P0AG59) Pfam (PF00253) | Escherichia coli | |
| 32 | HA (T) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (D7XN21) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4) | Escherichia coli | |
| 33 | IA (U) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (B7MIU7) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3) | Escherichia coli | |
| 34 | JA (V) | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (A0A080IK26) Pfam (PF00366) UniProt (by SIFTS) (P0AG63) | Escherichia coli | |
| 35 | KA (W) | 30S ribosomal protein S19 | polymer | 83 | 9421.0 | 1 | UniProt (S1EA57) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3) | Escherichia coli | |
| 36 | LA (X) | 30S ribosomal protein S13 | polymer | 116 | 12868.1 | 1 | UniProt (I2X719) UniProt (by SIFTS) (P0A7S9) Pfam (PF00416) | Escherichia coli | |
| 37 | MA (Y) | 50S ribosomal protein L11 | polymer | 141 | 14763.2 | 1 | UniProt (I2WL52) UniProt (by SIFTS) (P0A7J7) Pfam (PF03946) Pfam (PF00298) | Escherichia coli | |
| 38 | NA (Z) | 50S ribosomal protein L7/L12 | polymer | 30 | 3300.9 | 1 | UniProt (A0A4C9D1M4) Pfam (PF16320) UniProt (by SIFTS) (P0A7K2) | Escherichia coli | |
| 39 | OA (a) | 23S rRNA | polymer | 2904 | 941635.4 | 1 | GenBank (937521852) | Escherichia coli | |
| 40 | PA (b) | 50S ribosomal protein L27 | polymer | 76 | 8275.5 | 1 | UniProt (A0A073FRE3) Pfam (PF01016) UniProt (by SIFTS) (P0A7L8) | Escherichia coli | |
| 41 | QA (c) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (A0A5B7P765) Pfam (PF00830) UniProt (by SIFTS) (P0A7M2) | Escherichia coli | |
| 42 | RA (d) | 5S rRNA | polymer | 120 | 38790.1 | 1 | GenBank (1273279017) | Escherichia coli | |
| 43 | SA (e) | 50S ribosomal protein L29 | polymer | 62 | 7155.3 | 1 | UniProt (I2X052) Pfam (PF00831) UniProt (by SIFTS) (P0A7M6) | Escherichia coli | |
| 44 | TA (f) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (A0A1X3K2S4) Pfam (PF00327) UniProt (by SIFTS) (P0AG51) | Escherichia coli | |
| 45 | UA (g) | 50S ribosomal protein L31 | polymer | 66 | 7516.7 | 1 | UniProt (V0YYV3) Pfam (PF01197) UniProt (by SIFTS) (P0A7M9) | Escherichia coli | |
| 46 | VA (h) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | Large ribosomal subunit protein uL2 |
| 47 | WA (i) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (H4J6G2) Pfam (PF01783) UniProt (by SIFTS) (P0A7N4) | Escherichia coli | |
| 48 | XA (j) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli | Large ribosomal subunit protein uL3 |
| 49 | YA (k) | 50S ribosomal protein L33 | polymer | 52 | 6057.2 | 1 | UniProt (T9DEE3) Pfam (PF00471) UniProt (by SIFTS) (P0A7N9) | Escherichia coli | |
| 50 | ZA (l) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (D7Z9F6) Pfam (PF00573) UniProt (by SIFTS) (P60723) | Escherichia coli | |
| 51 | AB (m) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (B7MGC4) Pfam (PF00468) UniProt (by SIFTS) (P0A7P5) | Escherichia coli | |
| 52 | BB (n) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (H4IXW7) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (P62399) | Escherichia coli | |
| 53 | CB (o) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (G0F552) UniProt (by SIFTS) (P0A7Q1) Pfam (PF01632) | Escherichia coli | |
| 54 | DB (p) | 50S ribosomal protein L6 | polymer | 175 | 18672.4 | 1 | UniProt (A0A5B7MHD1) UniProt (by SIFTS) (P0AG55) Pfam (PF00347) | Escherichia coli | |
| 55 | EB (q) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A0E2L017) UniProt (by SIFTS) (P0A7Q6) Pfam (PF00444) | Escherichia coli | |
| 56 | FB (r) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (D7ZI15) UniProt (by SIFTS) (P0A7R1) Pfam (PF01281) Pfam (PF03948) | Escherichia coli | |
| 57 | GB (s) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (D7ZET0) UniProt (by SIFTS) (P0AA10) Pfam (PF00572) | Escherichia coli | |
| 58 | HB (t) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (D7Z9G5) UniProt (by SIFTS) (P0ADY3) Pfam (PF00238) | Escherichia coli | |
| 59 | IB (u) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A037Y8L6) UniProt (by SIFTS) (P02413) Pfam (PF00828) | Escherichia coli | |
| 60 | JB (v) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 61 | KB (w) | 50S ribosomal protein L17 | polymer | 119 | 13592.8 | 1 | UniProt (A0A2T1LEK9) UniProt (by SIFTS) (P0AG44) Pfam (PF01196) | Escherichia coli | |
| 62 | LB (x) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (F4SQ31) UniProt (by SIFTS) (P0C018) Pfam (PF00861) | Escherichia coli | |
| 63 | MB (y) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (I2XAW2) UniProt (by SIFTS) (P0A7K6) Pfam (PF01245) | Escherichia coli | |
| 64 | NB (z) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (A0A7B6WTL0) Pfam (PF00453) UniProt (by SIFTS) (P0A7L3) | Escherichia coli | |
| 65 | OB (7) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 66 | PB, QB (AA) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
AE: 1 - 1407 (UniProt: A0A4S1NBU2)
a: 1 - 2904 (GenBank: 937521852)
| PDB | External Database | Details |
|---|---|---|
| Val 1384 | Met 1384 | conflict |
| PDB | External Database | Details |
|---|---|---|
| A 887 | U 472128 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 66 |
| Total formula weight | 2667169.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 2667324.9 |
