6VU3
Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | 0 | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (D7ZEN7) UniProt (by SIFTS) (P0AG48) Pfam (PF00829) In PDB | Escherichia coli | |
2 | 1 | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (D7Z9G0) UniProt (by SIFTS) (P61175) Pfam (PF00237) In PDB | Escherichia coli | |
3 | 2 | 50S ribosomal protein L23 | polymer | 94 | 10661.6 | 1 | UniProt (D6I208) UniProt (by SIFTS) (P0ADZ0) Pfam (PF00276) In PDB | Escherichia coli | |
4 | 3 | 50S ribosomal protein L24 | polymer | 103 | 11208.1 | 1 | UniProt (D7XKZ6) UniProt (by SIFTS) (P60624) Pfam (PF00467) Pfam (PF17136) In PDB | Escherichia coli | |
5 | 4 | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (D7XH79) UniProt (by SIFTS) (P68919) Pfam (PF01386) In PDB | Escherichia coli | |
6 | 5 | NT DNA | polymer | 36 | 11042.1 | 1 | synthetic construct | ||
7 | 6 | T DNA | polymer | 27 | 8155.2 | 1 | synthetic construct | ||
8 | 7 | mRNA with 12 nt long spacer | polymer | 29 | 9238.3 | 1 | synthetic construct | ||
9 | 9 | 50S ribosomal protein L10 | polymer | 148 | 15981.5 | 1 | UniProt (A0A3W3FJZ4) UniProt (by SIFTS) (P0A7J3) Pfam (PF00466) In PDB | Escherichia coli | |
10 | A, B | E-site and A-site tRNA (fMet) | polymer | 76 | 24496.6 | 2 | GenBank (1845826323) | Escherichia coli | |
11 | AA | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (A0A140SS80) UniProt (by SIFTS) (P0A8V2) Pfam (PF04561) Pfam (PF04563) Pfam (PF10385) Pfam (PF04565) Pfam (PF00562) Pfam (PF04560) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
12 | AB | Transcription termination/antitermination protein NusG | polymer | 181 | 20560.5 | 1 | UniProt (U9XYQ6) UniProt (by SIFTS) (P0AFG0) Pfam (PF02357) Pfam (PF00467) In PDB | Escherichia coli | |
13 | AC, AD | DNA-directed RNA polymerase subunit alpha | polymer | 230 | 25497.0 | 2 | UniProt (A0A5F0PYH4) UniProt (by SIFTS) (P0A7Z4) Pfam (PF01193) Pfam (PF01000) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
14 | AE | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) UniProt (by SIFTS) (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
15 | C | 30S ribosomal protein S18 | polymer | 66 | 7734.9 | 1 | UniProt (A0A222QU44) UniProt (by SIFTS) (P0A7T7) Pfam (PF01084) In PDB | Escherichia coli | |
16 | D | 16S rRNA | polymer | 1542 | 499690.0 | 1 | GenBank (1732209707) | Escherichia coli | |
17 | E | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (D7X3A7) UniProt (by SIFTS) (P0A7U7) Pfam (PF01649) In PDB | Escherichia coli | |
18 | F | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (E9TH65) UniProt (by SIFTS) (P68679) Pfam (PF01165) In PDB | Escherichia coli | |
19 | G | 30S ribosomal protein S2 | polymer | 225 | 25072.9 | 1 | UniProt (C3TPN2) UniProt (by SIFTS) (P0A7V0) Pfam (PF00318) In PDB | Escherichia coli | |
20 | H | 30S ribosomal protein S1 | polymer | 557 | 61238.9 | 1 | UniProt (B7LDA3) UniProt (by SIFTS) (P0AG67) Pfam (PF00575) In PDB | Escherichia coli | |
21 | I | 30S ribosomal protein S3 | polymer | 208 | 23249.0 | 1 | UniProt (A0A376HTV6) UniProt (by SIFTS) (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB | Escherichia coli | |
22 | J | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (L3PZ69) UniProt (by SIFTS) (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB | Escherichia coli | |
23 | K | 30S ribosomal protein S5 | polymer | 156 | 16475.0 | 1 | UniProt (F4TL26) UniProt (by SIFTS) (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB | Escherichia coli | |
24 | L | 30S ribosomal protein S6 | polymer | 104 | 12126.0 | 1 | UniProt (A0A376ZL25) UniProt (by SIFTS) (P02358) Pfam (PF01250) In PDB | Escherichia coli | |
25 | M | 30S ribosomal protein S7 | polymer | 151 | 16861.5 | 1 | UniProt (S1D5F0) UniProt (by SIFTS) (P02359) Pfam (PF00177) In PDB | Escherichia coli | |
26 | N | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (D8A1L7) UniProt (by SIFTS) (P0A7W7) Pfam (PF00410) In PDB | Escherichia coli | |
27 | O | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (T9TH92) UniProt (by SIFTS) (P0A7X3) Pfam (PF00380) In PDB | Escherichia coli | |
28 | P | 30S ribosomal protein S10 | polymer | 99 | 11254.0 | 1 | UniProt (A0A069XEJ0) UniProt (by SIFTS) (P0A7R5) Pfam (PF00338) In PDB | Escherichia coli | |
29 | Q | 30S ribosomal protein S11 | polymer | 117 | 12487.2 | 1 | UniProt (P0A7R9) Pfam (PF00411) In PDB | Escherichia coli | Small ribosomal subunit protein uS11 |
30 | R | 30S ribosomal protein S12 | polymer | 124 | 13768.2 | 1 | UniProt (I2UHF1) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3) In PDB | Escherichia coli | |
31 | S | 30S ribosomal protein S14 | polymer | 100 | 11475.4 | 1 | UniProt (I2X0X3) UniProt (by SIFTS) (P0AG59) Pfam (PF00253) In PDB | Escherichia coli | |
32 | T | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (D7XN21) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4) In PDB | Escherichia coli | |
33 | U | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (B7MIU7) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3) In PDB | Escherichia coli | |
34 | V | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (A0A080IK26) Pfam (PF00366) UniProt (by SIFTS) (P0AG63) In PDB | Escherichia coli | |
35 | W | 30S ribosomal protein S19 | polymer | 83 | 9421.0 | 1 | UniProt (S1EA57) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3) In PDB | Escherichia coli | |
36 | X | 30S ribosomal protein S13 | polymer | 116 | 12868.1 | 1 | UniProt (A0A4P8BX31) UniProt (by SIFTS) (P0A7S9) Pfam (PF00416) In PDB | Escherichia coli | |
37 | Y | 50S ribosomal protein L11 | polymer | 141 | 14763.2 | 1 | UniProt (S1ERR4) UniProt (by SIFTS) (P0A7J7) Pfam (PF03946) Pfam (PF00298) In PDB | Escherichia coli | |
38 | Z | 50S ribosomal protein L7/L12 | polymer | 30 | 3300.9 | 1 | UniProt (A0A376CUC4) Pfam (PF16320) UniProt (by SIFTS) (P0A7K2) In PDB | Escherichia coli | |
39 | a | 23S rRNA | polymer | 2904 | 941635.4 | 1 | GenBank (937521852) | Escherichia coli | |
40 | b | 50S ribosomal protein L27 | polymer | 76 | 8275.5 | 1 | UniProt (A0A069XRX8) Pfam (PF01016) UniProt (by SIFTS) (P0A7L8) In PDB | Escherichia coli | |
41 | c | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (J7RMJ2) Pfam (PF00830) UniProt (by SIFTS) (P0A7M2) In PDB | Escherichia coli | |
42 | d | 5S rRNA | polymer | 120 | 38790.1 | 1 | GenBank (1273279017) | Escherichia coli | |
43 | e | 50S ribosomal protein L29 | polymer | 62 | 7155.3 | 1 | UniProt (A0A069XF36) Pfam (PF00831) UniProt (by SIFTS) (P0A7M6) In PDB | Escherichia coli | |
44 | f | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (I2X1N4) Pfam (PF00327) UniProt (by SIFTS) (P0AG51) In PDB | Escherichia coli | |
45 | g | 50S ribosomal protein L31 | polymer | 66 | 7516.7 | 1 | UniProt (V0YYV3) Pfam (PF01197) UniProt (by SIFTS) (P0A7M9) In PDB | Escherichia coli | |
46 | h | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB | Escherichia coli | Large ribosomal subunit protein uL2 |
47 | i | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (E3PJQ3) Pfam (PF01783) UniProt (by SIFTS) (P0A7N4) In PDB | Escherichia coli | |
48 | j | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) In PDB | Escherichia coli | Large ribosomal subunit protein uL3 |
49 | k | 50S ribosomal protein L33 | polymer | 52 | 6057.2 | 1 | UniProt (L4IX08) Pfam (PF00471) UniProt (by SIFTS) (P0A7N9) In PDB | Escherichia coli | |
50 | l | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (D7Z9F6) Pfam (PF00573) UniProt (by SIFTS) (P60723) In PDB | Escherichia coli | |
51 | m | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (B7MGC4) Pfam (PF00468) UniProt (by SIFTS) (P0A7P5) In PDB | Escherichia coli | |
52 | n | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (S1EUW9) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (P62399) In PDB | Escherichia coli | |
53 | o | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (G0F552) UniProt (by SIFTS) (P0A7Q1) Pfam (PF01632) In PDB | Escherichia coli | |
54 | p | 50S ribosomal protein L6 | polymer | 175 | 18672.4 | 1 | UniProt (A0A5B7PA13) UniProt (by SIFTS) (P0AG55) Pfam (PF00347) In PDB | Escherichia coli | |
55 | q | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A0E2L017) UniProt (by SIFTS) (P0A7Q6) Pfam (PF00444) In PDB | Escherichia coli | |
56 | r | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (D7ZI15) UniProt (by SIFTS) (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB | Escherichia coli | |
57 | s | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (D7ZET0) UniProt (by SIFTS) (P0AA10) Pfam (PF00572) In PDB | Escherichia coli | |
58 | t | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (D7Z9G5) UniProt (by SIFTS) (P0ADY3) Pfam (PF00238) In PDB | Escherichia coli | |
59 | u | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A037Y8L6) UniProt (by SIFTS) (P02413) Pfam (PF00828) In PDB | Escherichia coli | |
60 | v | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) In PDB | Escherichia coli | Large ribosomal subunit protein uL16 |
61 | w | 50S ribosomal protein L17 | polymer | 119 | 13592.8 | 1 | UniProt (A0A2T1LEK9) UniProt (by SIFTS) (P0AG44) Pfam (PF01196) In PDB | Escherichia coli | |
62 | x | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (F4TL27) UniProt (by SIFTS) (P0C018) Pfam (PF00861) In PDB | Escherichia coli | |
63 | y | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (A0A4P8C2I6) UniProt (by SIFTS) (P0A7K6) Pfam (PF01245) In PDB | Escherichia coli | |
64 | z | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (L3CCT0) Pfam (PF00453) UniProt (by SIFTS) (P0A7L3) In PDB | Escherichia coli | |
65 | AE | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
66 | AE | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 76 (GenBank: 1845826323)
AE: 1 - 1407 (UniProt: A0A4S1NBU2)
a: 1 - 2904 (GenBank: 937521852)
PDB | External Database | Details |
---|---|---|
- | U 634153 | deletion |
PDB | External Database | Details |
---|---|---|
Val 1384 | Met 1384 | variant |
PDB | External Database | Details |
---|---|---|
A 887 | U 472128 | variant |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 66 |
Total formula weight | 2667169.8 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 2667324.9 |