6VSP

Structure of Serratia marcescens 2,3-butanediol dehydrogenase mutant Q247A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	2,3-butanediol dehydrogenase	polymer	264	27882.7	2	UniProt (H9XP47) Pfam (PF13561)	Serratia marcescens	3-oxoacyl-ACP reductase,SDR family oxidoreductase
2	C, D (A)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G, H, I, L... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
5	J, O (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	K (B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7	P, Q (A, B)	water	water		18.0	539	Chemie (HOH)

Sequence modifications

A, B: 2 - 251 (UniProt: H9XP47)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Ala 247	Gln 247	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55765.4
Non-Polymers*	Number of molecules	13
	Total formula weight	2326.6
All*	Total formula weight	58092.0

*Water molecules are not included.