6VSJ

Cryo-electron microscopy structure of mouse coronavirus spike protein complexed with its murine receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, B, C)	Spike glycoprotein	polymer	1275	141118.8	3	UniProt (P11224) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Murine coronavirus (strain A59) (MHV-A59)	S glycoprotein,E2,Peplomer protein
2	B, D, F (D, E, F)	Carcinoembryonic antigen-related cell adhesion molecule 1	polymer	208	23678.7	3	UniProt (P31809) Pfam (PF07686) Pfam (PF13927)	Mus musculus (Mouse)	Biliary glycoprotein 1,BGP-1,Biliary glycoprotein D,MHVR1,Murine hepatitis virus receptor,MHV-R
3	G, H, I, J, K... (A, D, B, E, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)

Sequence modifications

A, B, C: 15 - 1228 (UniProt: P11224)

PDB	External Database	Details
Met -6	-	initiating methionine
Lys -5	-	expression tag
Phe -4	-	expression tag
Leu -3	-	expression tag
Val -2	-	expression tag
Asn -1	-	expression tag
Val 0	-	expression tag
Ala 1	-	expression tag
Leu 2	-	expression tag
Val 3	-	expression tag
Phe 4	-	expression tag
Met 5	-	expression tag
Val 6	-	expression tag
Val 7	-	expression tag
Tyr 8	-	expression tag
Ile 9	-	expression tag
Ser 10	-	expression tag
Tyr 11	-	expression tag
Ile 12	-	expression tag
Tyr 13	-	expression tag
Ala 14	-	expression tag
Phe 478	Tyr 478	conflict
Ile 1229	-	expression tag
Lys 1230	-	expression tag
Arg 1231	-	expression tag
Met 1232	-	expression tag
Lys 1233	-	expression tag
Gln 1234	-	expression tag
Ile 1235	-	expression tag
Glu 1236	-	expression tag
Asp 1237	-	expression tag
Lys 1238	-	expression tag
Ile 1239	-	expression tag
Glu 1240	-	expression tag
Glu 1241	-	expression tag
Ile 1242	-	expression tag
Glu 1243	-	expression tag
Ser 1244	-	expression tag
Lys 1245	-	expression tag
Gln 1246	-	expression tag
Lys 1247	-	expression tag
Lys 1248	-	expression tag
Ile 1249	-	expression tag
Glu 1250	-	expression tag
Asn 1251	-	expression tag
Glu 1252	-	expression tag
Ile 1253	-	expression tag
Ala 1254	-	expression tag
Arg 1255	-	expression tag
Ile 1256	-	expression tag
Lys 1257	-	expression tag
Lys 1258	-	expression tag
Ile 1259	-	expression tag
Lys 1260	-	expression tag
His 1261	-	expression tag
His 1262	-	expression tag
His 1263	-	expression tag
His 1264	-	expression tag
His 1265	-	expression tag
His 1266	-	expression tag
His 1267	-	expression tag
His 1268	-	expression tag

D, E, F: 35 - 236 (UniProt: P31809)

PDB	External Database	Details
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	494392.5
Non-Polymers*	Number of molecules	12
	Total formula weight	2654.5
All*	Total formula weight	497047.0

*Water molecules are not included.