6VQW

Type I-F CRISPR-Csy complex with its inhibitor AcrF8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Type I-F CRISPR-associated protein Csy2	polymer	327	36244.1	1	UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB	Pseudomonas aeruginosa	Csy2 (Cas5f)
2	D, E, F, G, H...	CRISPR-associated protein Csy3	polymer	360	39778.6	6	UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB	Pseudomonas aeruginosa	Csy3 (Cas7f)
3	J	CRISPR-associated endonuclease Cas6/Csy4	polymer	187	21427.5	1	UniProt (Q02MM2) Pfam (PF09618) In PDB	Pseudomonas aeruginosa	Csy4 (Cas6f)
4	A	AcrF8	polymer	92	10206.4	1		Pseudomonas aeruginosa
5	B	CRISPR-associated protein Csy1	polymer	434	49194.2	1	UniProt (Q02ML9) Pfam (PF09611) In PDB	Pseudomonas aeruginosa	Csy1 (Cas8f)
6	K	CrRNA (40-MER)	polymer	60	19249.4	1	GenBank (313291946)	Pseudomonas aeruginosa

Sequence modifications

D, E, F, G, H, I: 20 - 360 (UniProt: A0A444M080)

PDB	External Database	Details
Met 1	-	expression tag
Lys 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Glu 11	-	expression tag
Asn 12	-	expression tag
Leu 13	-	expression tag
Tyr 14	-	expression tag
Phe 15	-	expression tag
Gln 16	-	expression tag
Ser 17	-	expression tag
Asn 18	-	expression tag
Ala 19	-	expression tag

K: 1 - 60 (GenBank: 313291946)

PDB	External Database	Details
A 53	G 1446	conflict

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	374993.1
All*	Total formula weight	374993.1

*Water molecules are not included.