6VOY

Cryo-EM structure of HTLV-1 instasome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	DNA-binding protein 7d	polymer	390	43652.7	4	UniProt (P39476) UniProt (A0A1Y1CAW1) Pfam (PF02294) Pfam (PF02022) Pfam (PF00665) Pfam (PF00552) UniProt (by SIFTS) (P14078)	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	7 kDa DNA-binding protein d,Sso7d
2	E, F (E, F)	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform	polymer	343	40357.8	2	UniProt (Q13362) Pfam (PF01603)	Homo sapiens (Human)	PP2A B subunit isoform B'-gamma,PP2A B subunit isoform B56-gamma,PP2A B subunit isoform PR61-gamma,PP2A B subunit isoform R5-gamma,Renal carcinoma antigen NY-REN-29
3	G, J (I, L)	DNA (5'-D(P*AP*CP*AP*CP*AP*CP*TP*TP*GP*AP*CP*TP*AP*GP*GP*GP*TP*G)-3')	polymer	49	15074.7	2		Homo sapiens
4	H, K (J, M)	DNA (25-MER)	polymer	25	7614.9	2		Human T-cell leukemia virus type I
5	I, L (K, N)	DNA (5'-D(P*AP*CP*AP*CP*AP*CP*TP*TP*GP*AP*CP*TP*AP*GP*GP*GP*TP*G)-3')	polymer	20	6199.0	2		Human T-cell leukemia virus type I
6	M, O, P, R (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	N, Q (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B, C, D: -74 - -11 (UniProt: P39476)

PDB	External Database	Details
Met -94	-	expression tag
Gly -93	-	expression tag
Ser -92	-	expression tag
Ser -91	-	expression tag
His -90	-	expression tag
His -89	-	expression tag
His -88	-	expression tag
His -87	-	expression tag
His -86	-	expression tag
His -85	-	expression tag
Ser -84	-	expression tag
Ser -83	-	expression tag
Gly -82	-	expression tag
Leu -81	-	expression tag
Val -80	-	expression tag
Pro -79	-	expression tag
Arg -78	-	expression tag
Gly -77	-	expression tag
Ser -76	-	expression tag
His -75	-	expression tag
Ala -51	Trp 24	engineered mutation
Glu -32	Arg 43	conflict
Gly -10	-	linker
Gly -9	-	linker
Ser -8	-	linker
Leu -7	-	linker
Glu -6	-	linker
Val -5	-	linker
Leu -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Pro 0	-	linker

A, B, C, D: 1 - 295 (UniProt: A0A1Y1CAW1)

PDB	External Database	Details
Gln 156	Glu 724	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	313103.6
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	313413.8

*Water molecules are not included.