6VCL
Crystal structure of E.coli RppH-DapF in complex with pppGpp, Mg2+ and F-
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Diaminopimelate epimerase | polymer | 275 | 30154.4 | 1 | UniProt (P0A6K1) Pfam (PF01678) | Escherichia coli (strain K12) | DAP epimerase,PLP-independent amino acid racemase |
| 2 | B (B) | RNA pyrophosphohydrolase | polymer | 161 | 18965.8 | 1 | UniProt (P0A776) Pfam (PF00293) | Escherichia coli (strain K12) | (Di)nucleoside polyphosphate hydrolase,Ap5A pyrophosphatase |
| 3 | C, J (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 4 | D, E, F, G (B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 5 | H, I (B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | K (B) | FLUORIDE ION | non-polymer | 19.0 | 1 | Chemie (F) | |||
| 7 | L (B) | guanosine 5'-(tetrahydrogen triphosphate) 3'-(trihydrogen diphosphate) | non-polymer | 683.1 | 1 | Chemie (0O2) | |||
| 8 | M, N (A, B) | water | water | 18.0 | 280 | Chemie (HOH) |
Sequence modifications
A: 1 - 274 (UniProt: P0A6K1)
B: 1 - 160 (UniProt: P0A776)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 36 | Arg 36 | engineered mutation |
| Ala 268 | Tyr 268 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 159 | Gln 159 | engineered mutation |
| Ala 160 | Glu 160 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 49120.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1054.5 | |
| All* | Total formula weight | 50174.6 |
