6VCL

Crystal structure of E.coli RppH-DapF in complex with pppGpp, Mg2+ and F-

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Diaminopimelate epimerase	polymer	275	30154.4	1	UniProt (P0A6K1) Pfam (PF01678) In PDB	Escherichia coli (strain K12)	DAP epimerase,PLP-independent amino acid racemase
2	B	RNA pyrophosphohydrolase	polymer	161	18965.8	1	UniProt (P0A776) Pfam (PF00293) In PDB	Escherichia coli (strain K12)	(Di)nucleoside polyphosphate hydrolase,Ap5A pyrophosphatase
3	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	B	FLUORIDE ION	non-polymer		19.0	1	Chemie (F)
7	B	guanosine 5'-(tetrahydrogen triphosphate) 3'-(trihydrogen diphosphate)	non-polymer		683.1	1	Chemie (0O2)
8		water	water		18.0	280	Chemie (HOH)

Sequence modifications

A: 1 - 274 (UniProt: P0A6K1)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 36	Arg 36	engineered mutation
Ala 268	Tyr 268	engineered mutation

B: 1 - 160 (UniProt: P0A776)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 159	Gln 159	engineered mutation
Ala 160	Glu 160	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49120.2
Non-Polymers*	Number of molecules	10
	Total formula weight	1054.5
All*	Total formula weight	50174.6

*Water molecules are not included.