6VCK

Crystal structure of E.coli RppH-DapF in complex with GDP, Mg2+ and F-

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Diaminopimelate epimerase	polymer	275	30154.4	1	UniProt (P0A6K1) Pfam (PF01678) In PDB	Escherichia coli (strain K12)	DAP epimerase,PLP-independent amino acid racemase
2	B	RNA pyrophosphohydrolase	polymer	161	18965.8	1	UniProt (P0A776) Pfam (PF00293) In PDB	Escherichia coli (strain K12)	(Di)nucleoside polyphosphate hydrolase,Ap5A pyrophosphatase
3	B	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
5	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	B	FLUORIDE ION	non-polymer		19.0	1	Chemie (F)
7		water	water		18.0	102	Chemie (HOH)

Sequence modifications

A: 1 - 274 (UniProt: P0A6K1)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 36	Arg 36	engineered mutation
Ala 268	Tyr 268	engineered mutation

B: 1 - 160 (UniProt: P0A776)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 159	Gln 159	engineered mutation
Ala 160	Glu 160	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49120.2
Non-Polymers*	Number of molecules	6
	Total formula weight	570.6
All*	Total formula weight	49690.8

*Water molecules are not included.