6VCB
Cryo-EM structure of the Glucagon-like peptide-1 receptor in complex with G protein, GLP-1 peptide and a positive allosteric modulator
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Glucagon-like peptide 1 receptor | polymer | 445 | 51233.1 | 1 | UniProt (P43220) Pfam (PF02793) Pfam (PF00002) | Homo sapiens | GLP-1R |
| 2 | B (P) | Glucagon-like peptide 1 | polymer | 31 | 3359.7 | 1 | UniProt (P01275) Pfam (PF00123) | Homo sapiens | |
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44326.2 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 350 | 38534.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 6 | F (N) | Nanobody 35 | polymer | 160 | 17352.5 | 1 | Lama glama (llama) | ||
| 7 | G (R) | 1-[(1R)-1-(2,6-dichloro-3-methoxyphenyl)ethyl]-6-{2-[(2R)-piperidin-2-yl]phenyl}-1H-benzimidazole | non-polymer | 480.4 | 1 | Chemie (QW7) |
Sequence modifications
R: 24 - 422 (UniProt: P43220)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| Lys -21 | - | expression tag |
| Thr -20 | - | expression tag |
| Ile -19 | - | expression tag |
| Ile -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Leu -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Tyr -14 | - | expression tag |
| Ile -13 | - | expression tag |
| Phe -12 | - | expression tag |
| Cys -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Val -9 | - | expression tag |
| Phe -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Ala 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Gly 5 | - | expression tag |
| Ser 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Leu 10 | - | expression tag |
| Glu 11 | - | expression tag |
| Val 12 | - | expression tag |
| Leu 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Gln 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Gly 19 | - | expression tag |
| Ser 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Gly 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Phe 260 | Leu 260 | variant |
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 162666.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 480.4 | |
| All* | Total formula weight | 163147.1 |
