6VBJ

CRYSTAL STRUCTURE OF THE HYBRID C-TERMINAL DOMAIN OF ENZYME I OF THE BACTERIAL PHOSPHOTRANSFERASE SYSTEM FORMED BY HYBRIDIZING THE SCAFFOLD OF THE THERMOANAEROBACTER TENGCONGENSIS ENZYME WITH THE ACTIVE SITE LOOPS FROM THE ESCHERICHIA COLI ENZYME

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Phosphoenolpyruvate-protein phosphotransferase	polymer	314	34891.4	2	UniProt (Q8R7R4) Pfam (PF02896)	Caldanaerobacter subterraneus subsp. tengcongensis	Phosphotransferase system,enzyme I
2	C, D (A, B)	water	water		18.0	433	Chemie (HOH)

Sequence modifications

A, B: 261 - 573 (UniProt: Q8R7R4)

PDB	External Database	Details
Met 260	-	initiating methionine
Val 278	Pro 278	engineered mutation
Arg 279	Lys 279	engineered mutation
Phe 301	Tyr 301	engineered mutation
Asp 305	Asn 305	engineered mutation
Ala 306	Ser 306	engineered mutation
Thr 309	Ser 309	engineered mutation
Met 334	Leu 334	engineered mutation
Met 345	Leu 345	engineered mutation
Asn 346	Asp 346	engineered mutation
Phe 347	Met 347	engineered mutation
Glu 351	Met 351	engineered mutation
Trp 357	Tyr 357	engineered mutation
Gly 466	Met 466	engineered mutation
Asp 468	Glu 468	engineered mutation
Met 469	His 469	engineered mutation
Ile 470	Val 470	engineered mutation
Ser 471	Lys 471	engineered mutation
His 472	Glu 472	engineered mutation
Leu 473	Tyr 473	engineered mutation
Met 477	Phe 477	engineered mutation
Ser 478	His 478	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69782.8
All*	Total formula weight	69782.8

*Water molecules are not included.