6V2B

Complex of double mutant (T89V,K162T) of E. coli L-asparaginase II with L-Asn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L-asparaginase 2	polymer	333	35556.8	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A, B, C, D	ASPARAGINE	non-polymer		132.1	4	Chemie (ASN)
3	A, C, D	CITRIC ACID	non-polymer		192.1	3	Chemie (CIT)
4		water	water		18.0	679	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Val 89	Thr 111	engineered mutation
Thr 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142227.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1104.8
All*	Total formula weight	143332.2

*Water molecules are not included.