6V05
Cryo-EM structure of a substrate-engaged Bam complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Outer membrane protein assembly factor BamA | polymer | 810 | 90567.2 | 1 | UniProt (P0A940) Pfam (PF07244) Pfam (PF01103) In PDB | Escherichia coli (strain K12) | Omp85 |
2 | B | Outer membrane protein assembly factor BamB | polymer | 392 | 41918.9 | 1 | UniProt (P77774) Pfam (PF01011) Pfam (PF13360) In PDB | Escherichia coli (strain K12) | |
3 | C | Outer membrane protein assembly factor BamC | polymer | 344 | 36875.3 | 1 | UniProt (W8SZY2) Pfam (PF06804) UniProt (by SIFTS) (P0A903) In PDB | Escherichia coli (strain K12) | |
4 | D | Outer membrane protein assembly factor BamD | polymer | 245 | 27858.3 | 1 | UniProt (A0A0H3MJ38) Pfam (PF13525) UniProt (by SIFTS) (P0AC02) In PDB | Escherichia coli (strain K12) | |
5 | E | Outer membrane protein assembly factor BamE | polymer | 123 | 13530.3 | 1 | UniProt (A0A3S5E0E0) Pfam (PF04355) UniProt (by SIFTS) (P0A937) In PDB | Escherichia coli K-12 | |
6 | F | Outer membrane protein assembly factor BamA,Outer membrane protein assembly factor BamA,Outer membrane protein assembly factor BamA,Outer membrane protein assembly factor BamA | polymer | 578 | 64010.3 | 1 | UniProt (P0A940) Pfam (PF07244) Pfam (PF01103) In PDB | Escherichia coli (strain K12) | Omp85,Omp85,Omp85,Omp85 |
Sequence modifications
A: 1 - 810 (UniProt: P0A940)
E: 1 - 113 (UniProt: A0A3S5E0E0)
F: 233 - 253 (UniProt: P0A940)
F: 282 - 431 (UniProt: P0A940)
F: 432 - 439 (UniProt: P0A940)
F: 440 - 810 (UniProt: P0A940)
PDB | External Database | Details |
---|---|---|
Ser 690 | Cys 690 | engineered mutation |
Ser 700 | Cys 700 | engineered mutation |
Cys 804 | Phe 804 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 114 | - | expression tag |
Gly 115 | - | expression tag |
His 116 | - | expression tag |
His 117 | - | expression tag |
His 118 | - | expression tag |
His 119 | - | expression tag |
His 120 | - | expression tag |
His 121 | - | expression tag |
His 122 | - | expression tag |
His 123 | - | expression tag |
F: 282 - 431 (UniProt: P0A940)
PDB | External Database | Details |
---|---|---|
Trp 254 | - | linker |
Ser 255 | - | linker |
His 256 | - | linker |
Pro 257 | - | linker |
Gln 258 | - | linker |
Phe 259 | - | linker |
Glu 260 | - | linker |
Lys 261 | - | linker |
Gly 262 | - | linker |
Gly 263 | - | linker |
Gly 264 | - | linker |
Ser 265 | - | linker |
Gly 266 | - | linker |
Gly 267 | - | linker |
Gly 268 | - | linker |
Ser 269 | - | linker |
Gly 270 | - | linker |
Gly 271 | - | linker |
Ser 272 | - | linker |
Ala 273 | - | linker |
Trp 274 | - | linker |
Ser 275 | - | linker |
His 276 | - | linker |
Pro 277 | - | linker |
Gln 278 | - | linker |
Phe 279 | - | linker |
Glu 280 | - | linker |
Lys 281 | - | linker |
F: 440 - 810 (UniProt: P0A940)
PDB | External Database | Details |
---|---|---|
Cys 467 | Thr 467 | engineered mutation |
Ser 690 | Cys 690 | engineered mutation |
Ser 700 | Cys 700 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 274760.3 | |
All* | Total formula weight | 274760.3 |