6V05

Cryo-EM structure of a substrate-engaged Bam complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	810	90567.2	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli (strain K12)	Omp85
2	B (B)	Outer membrane protein assembly factor BamB	polymer	392	41918.9	1	UniProt (P77774) Pfam (PF01011) Pfam (PF13360)	Escherichia coli (strain K12)
3	C (C)	Outer membrane protein assembly factor BamC	polymer	344	36875.3	1	UniProt (W8SZY2) Pfam (PF06804) UniProt (by SIFTS) (P0A903)	Escherichia coli (strain K12)
4	D (D)	Outer membrane protein assembly factor BamD	polymer	245	27858.3	1	UniProt (A0A0H3MJ38) Pfam (PF13525) UniProt (by SIFTS) (P0AC02)	Escherichia coli (strain K12)
5	E (E)	Outer membrane protein assembly factor BamE	polymer	123	13530.3	1	UniProt (A0A3S5E0E0) Pfam (PF04355) UniProt (by SIFTS) (P0A937)	Escherichia coli K-12
6	F (F)	Outer membrane protein assembly factor BamA,Outer membrane protein assembly factor BamA,Outer membrane protein assembly factor BamA,Outer membrane protein assembly factor BamA	polymer	578	64010.3	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli (strain K12)	Omp85,Omp85,Omp85,Omp85

Sequence modifications

A: 1 - 810 (UniProt: P0A940)

PDB	External Database	Details
Ser 690	Cys 690	engineered mutation
Ser 700	Cys 700	engineered mutation
Cys 804	Phe 804	engineered mutation

E: 1 - 113 (UniProt: A0A3S5E0E0)

PDB	External Database	Details
Gly 114	-	expression tag
Gly 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

F: 233 - 253 (UniProt: P0A940)
F: 282 - 431 (UniProt: P0A940)

PDB	External Database	Details
Trp 254	-	linker
Ser 255	-	linker
His 256	-	linker
Pro 257	-	linker
Gln 258	-	linker
Phe 259	-	linker
Glu 260	-	linker
Lys 261	-	linker
Gly 262	-	linker
Gly 263	-	linker
Gly 264	-	linker
Ser 265	-	linker
Gly 266	-	linker
Gly 267	-	linker
Gly 268	-	linker
Ser 269	-	linker
Gly 270	-	linker
Gly 271	-	linker
Ser 272	-	linker
Ala 273	-	linker
Trp 274	-	linker
Ser 275	-	linker
His 276	-	linker
Pro 277	-	linker
Gln 278	-	linker
Phe 279	-	linker
Glu 280	-	linker
Lys 281	-	linker

F: 432 - 439 (UniProt: P0A940)
F: 440 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 467	Thr 467	engineered mutation
Ser 690	Cys 690	engineered mutation
Ser 700	Cys 700	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	274760.3
All*	Total formula weight	274760.3

*Water molecules are not included.