6UUN

CryoEM Structure of the active Adrenomedullin 1 receptor G protein complex with adrenomedullin peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	E	Receptor activity-modifying protein 2	polymer	156	17839.4	1	UniProt (O60895) Pfam (PF04901) In PDB	Homo sapiens (Human)	Calcitonin-receptor-like receptor activity-modifying protein 2,CRLR activity-modifying protein 2
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)
5	N	Nanobody 35	polymer	138	15140.7	1		Lama glama (llama)
6	P	ADM	polymer	53	6038.8	1	UniProt (P35318) Pfam (PF00214) In PDB	Homo sapiens
7	R	Calcitonin gene-related peptide type 1 receptor	polymer	490	56274.5	1	UniProt (Q16602) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	CGRP type 1 receptor,Calcitonin receptor-like receptor

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

E: 44 - 175 (UniProt: O60895)

PDB	External Database	Details
Met 20	-	initiating methionine
Lys 21	-	expression tag
Thr 22	-	expression tag
Ile 23	-	expression tag
Ile 24	-	expression tag
Ala 25	-	expression tag
Leu 26	-	expression tag
Ser 27	-	expression tag
Tyr 28	-	expression tag
Ile 29	-	expression tag
Phe 30	-	expression tag
Cys 31	-	expression tag
Leu 32	-	expression tag
Val 33	-	expression tag
Phe 34	-	expression tag
Ala 35	-	expression tag
Asp 36	-	expression tag
Tyr 37	-	expression tag
Lys 38	-	expression tag
Asp 39	-	expression tag
Asp 40	-	expression tag
Asp 41	-	expression tag
Asp 42	-	expression tag
Lys 43	-	expression tag

P: 1 - 52 (UniProt: P35318)

PDB	External Database	Details
Nh2 53	-	amidation

R: 22 - 461 (UniProt: Q16602)

PDB	External Database	Details
Met -9	-	initiating methionine
Lys -8	-	expression tag
Thr -7	-	expression tag
Ile -6	-	expression tag
Ile -5	-	expression tag
Ala -4	-	expression tag
Leu -3	-	expression tag
Ser -2	-	expression tag
Tyr -1	-	expression tag
Ile 0	-	expression tag
Phe 1	-	expression tag
Cys 2	-	expression tag
Leu 3	-	expression tag
Val 4	-	expression tag
Phe 5	-	expression tag
Ala 6	-	expression tag
Asp 7	-	expression tag
Tyr 8	-	expression tag
Lys 9	-	expression tag
Asp 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Leu 14	-	expression tag
Glu 15	-	expression tag
Val 16	-	expression tag
Leu 17	-	expression tag
Phe 18	-	expression tag
Gln 19	-	expression tag
Gly 20	-	expression tag
Pro 21	-	expression tag
Pro 462	-	expression tag
Ala 463	-	expression tag
Gly 464	-	expression tag
Leu 465	-	expression tag
Glu 466	-	expression tag
Val 467	-	expression tag
Leu 468	-	expression tag
Phe 469	-	expression tag
Gln 470	-	expression tag
Gly 471	-	expression tag
Pro 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	187372.1
All*	Total formula weight	187372.1

*Water molecules are not included.