6UU8
E. coli mutant sigma-S transcription initiation complex with a 7-nt RNA ("Fresh" mutant crystal soaked with GTP, UTP, and CTP for 30 minutes)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | AAA, BBB | DNA-directed RNA polymerase subunit alpha | polymer | 242 | 26899.6 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) In PDB | Escherichia coli | RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha |
2 | CCC | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | DDD | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
4 | EEE | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
5 | FFF | RNA polymerase sigma factor RpoS | polymer | 336 | 38705.5 | 1 | UniProt (A0A377K1M2) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P13445) In PDB | Escherichia coli | Sigma S,Sigma-38 |
6 | 111 | Synthetic DNA 50-mer (promoter non-template strand) | polymer | 50 | 15313.8 | 1 | synthetic construct | ||
7 | 222 | Synthetic DNA 50-mer (promoter template strand) | polymer | 50 | 15563.0 | 1 | synthetic construct | ||
8 | 333 | RNA 7-mer (de novo synthesized) | polymer | 7 | 2397.3 | 1 | synthetic construct | ||
9 | CCC, DDD | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
10 | DDD | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
11 | DDD | DIPHOSPHATE | non-polymer | 173.9 | 1 | Chemie (DPO) |
Sequence modifications
AAA, BBB: 1 - 235 (UniProt: P0A7Z4)
FFF: 1 - 328 (UniProt: A0A377K1M2)
PDB | External Database | Details |
---|---|---|
Ala -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 2 | Ser 2 | conflict |
Gly 219 | Ile 219 | engineered mutation |
Ala 221 | Ser 221 | engineered mutation |
Leu 329 | - | expression tag |
Glu 330 | - | expression tag |
His 331 | - | expression tag |
His 332 | - | expression tag |
His 333 | - | expression tag |
His 334 | - | expression tag |
His 335 | - | expression tag |
His 336 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 442084.8 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 353.4 | |
All* | Total formula weight | 442438.1 |