6UU8

E. coli mutant sigma-S transcription initiation complex with a 7-nt RNA ("Fresh" mutant crystal soaked with GTP, UTP, and CTP for 30 minutes)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, BBB	DNA-directed RNA polymerase subunit alpha	polymer	242	26899.6	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) In PDB	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	CCC	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	DDD	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	EEE	DNA-directed RNA polymerase subunit omega	polymer	90	10118.4	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	FFF	RNA polymerase sigma factor RpoS	polymer	336	38705.5	1	UniProt (A0A377K1M2) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P13445) In PDB	Escherichia coli	Sigma S,Sigma-38
6	111	Synthetic DNA 50-mer (promoter non-template strand)	polymer	50	15313.8	1		synthetic construct
7	222	Synthetic DNA 50-mer (promoter template strand)	polymer	50	15563.0	1		synthetic construct
8	333	RNA 7-mer (de novo synthesized)	polymer	7	2397.3	1		synthetic construct
9	CCC, DDD	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
10	DDD	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
11	DDD	DIPHOSPHATE	non-polymer		173.9	1	Chemie (DPO)

Sequence modifications

AAA, BBB: 1 - 235 (UniProt: P0A7Z4)

PDB	External Database	Details
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

FFF: 1 - 328 (UniProt: A0A377K1M2)

PDB	External Database	Details
Gly 2	Ser 2	conflict
Gly 219	Ile 219	engineered mutation
Ala 221	Ser 221	engineered mutation
Leu 329	-	expression tag
Glu 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	442084.8
Non-Polymers*	Number of molecules	5
	Total formula weight	353.4
All*	Total formula weight	442438.1

*Water molecules are not included.