6UU2
E. coli sigma-S transcription initiation complex with 3-nt RNA ("Old" crystal soaked with GTP and ATP for 30 minutes)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (AAA, BBB) | DNA-directed RNA polymerase subunit alpha | polymer | 242 | 26899.6 | 2 | UniProt (A0A377D9Q8) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | |
| 2 | C (CCC) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (DDD) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 4 | E (EEE) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
| 5 | F (FFF) | RNA polymerase sigma factor RpoS | polymer | 336 | 38777.6 | 1 | UniProt (P13445) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli (strain K12) | Sigma S,Sigma-38 |
| 6 | G (111) | Synthetic DNA 50-MER (promoter non-template strand) | polymer | 50 | 15313.8 | 1 | synthetic construct | ||
| 7 | H (222) | Synthethic DNA 50-MER (promoter template strand) | polymer | 50 | 15563.0 | 1 | synthetic construct | ||
| 8 | I (333) | RNA 3-mer | polymer | 3 | 1134.6 | 1 | synthetic construct | ||
| 9 | J, M (CCC, DDD) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 10 | K, L (DDD) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | N (DDD) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
AAA, BBB: 1 - 235 (UniProt: A0A377D9Q8)
FFF: 1 - 328 (UniProt: P13445)
| PDB | External Database | Details |
|---|---|---|
| Ala -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 2 | Ser 2 | conflict |
| Glu 33 | Gln 33 | conflict |
| Leu 329 | - | expression tag |
| Glu 330 | - | expression tag |
| His 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
| His 336 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 440894.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 702.6 | |
| All* | Total formula weight | 441596.8 |
