6UTH

Allosteric coupling between alpha-rings of 20S proteasome, 20S proteasome singly capped with a PA26/E102A_PANc, together with LFP incubation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Proteasome subunit alpha	polymer	227	25067.5	7	UniProt (P25156) Pfam (PF10584) Pfam (PF00227)	Thermoplasma acidophilum	20S proteasome alpha subunit,Proteasome core protein PsmA
2	H, I, J, K, L... (Z, M, 1, N, 2...)	Proteasome subunit beta	polymer	203	22294.8	14	UniProt (P28061) Pfam (PF00227)	Thermoplasma acidophilum	20S proteasome subunit B, 20S proteasome beta subunit, Proteasome core protein PsmB
3	AA, BA, V, W, X... (O, P, Q, R, S...)	Proteasome subunit alpha	polymer	227	25125.6	7	UniProt (P25156) Pfam (PF10584) Pfam (PF00227)	Thermoplasma acidophilum	20S proteasome alpha subunit,Proteasome core protein PsmA
4	CA, DA, EA, FA, GA... (a, b, c, d, e...)	Proteasome activator protein PA26	polymer	229	25088.6	7	UniProt (Q38BM8) Pfam (PF02252)	Trypanosoma brucei brucei

Sequence modifications

A, B, C, D, E, F, G: 7 - 233 (UniProt: P25156)

PDB	External Database	Details
Ala 66	Lys 66	conflict

a, b, c, d, e, f, g: 4 - 223 (UniProt: Q38BM8)

PDB	External Database	Details
Gly 48	Ala 48	conflict
Ala 102	Glu 102	conflict
Gly 224	-	expression tag
Gly 225	-	expression tag
His 226	-	expression tag
Leu 227	-	expression tag
Asp 228	-	expression tag
Val 229	-	expression tag
Leu 230	-	expression tag
Tyr 231	-	expression tag
Arg 232	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	35
	Total formula weight	839099.9
All*	Total formula weight	839099.9

*Water molecules are not included.