6USJ

Structure of two nucleosomes bridged by human PARP2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, K (I, S)	Widom 601 DNA (160-MER)	polymer	165	51141.6	2		synthetic construct
2	B, L (J, T)	Widom 601 DNA (160-MER)	polymer	165	50732.3	2		synthetic construct
3	C, G, M, Q (A, E, K, O)	Histone H3.1	polymer	139	15719.4	4	UniProt (P68431) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
4	D, H, N, R (B, F, L, P)	Histone H4	polymer	106	11676.7	4	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
5	E, I, O, S (C, G, M, Q)	Histone H2A	polymer	133	14447.8	4	UniProt (Q08AJ9) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P04908)	Homo sapiens (Human)
6	F, J, P, T (D, H, N, R)	Histone H2B type 1-J	polymer	129	14217.5	4	UniProt (P06899) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
7	U, V (U, V)	Poly [ADP-ribose] polymerase 2	polymer	590	67069.5	2	UniProt (Q9UGN5) Pfam (PF05406) Pfam (PF02877) Pfam (PF00644)	Homo sapiens (Human)	hPARP-2,ADP-ribosyltransferase diphtheria toxin-like 2,ARTD2,DNA ADP-ribosyltransferase PARP2,NAD(+) ADP-ribosyltransferase 2,ADPRT-2,Poly[ADP-ribose] synthase 2,pADPRT-2,Protein poly-ADP-ribosyltransferase PARP2

Sequence modifications

A, E, K, O: 0 - 135 (UniProt: P68431)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B, F, L, P: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G, M, Q: 0 - 129 (UniProt: Q08AJ9)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H, N, R: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

U, V: 1 - 570 (UniProt: Q9UGN5)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 112	Gln 112	engineered mutation
Asp 113	Phe 113	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	562132.6
All*	Total formula weight	562132.6

*Water molecules are not included.